Many biological processes are partitioned among organs and tissues, necessitating tissue-specific or organ-specific analysis (particularly for comparative -omics studies). Standardised techniques for tissue identification and dissection are therefore imperative for comparing among studies. Here we describe dissection protocols for isolating six key tissues/organs from larvae of the Asian longhorned beetle, Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae): the supraoesophageal ganglion, posterior midgut, hindgut, Malpighian tubules, fat body, and thoracic muscle. We also describe how to extract haemolymph and preserve whole larvae for measurements such as protein, lipid, and carbohydrate content. We include dissection protocols for both fresh-killed and previously frozen specimens. Although this protocol is developed for A. glabripennis, it should allow standardised tissue collection from larvae of other cerambycids and be readily transferrable to other beetle taxa with similar larval morphology.

许多生物学过程在器官和组织之间分配，需要进行组织特异性或器官特异性分析（特别是用于比较组学研究）。因此，用于研究之间比较的组织识别和解剖的标准化技术势在必行。在这里，我们描述了从亚洲长角甲虫（Anoplophora glabripennis）的幼虫中分离出六个关键组织/器官的解剖方法（Motschulsky）（鞘翅目：Cerambycidae）：食管上神经节，后中肠，后肠，马尔皮基小管，脂肪体和胸肌。我们还描述了如何提取血淋巴并保存整个幼虫以进行蛋白质，脂质和碳水化合物含量的测量。我们包括对新鲜杀死的和先前冷冻的标本的解剖方案。尽管此协议是为A. glabripennis开发的，但它应允许从其他陶瓷动物的幼虫中收集标准化的组织，并易于转移到具有相似幼虫形态的其他甲虫类群中。