Charge-conversional polyethylenimine-entrapped gold nanoparticles with 131I-labeling for enhanced dual mode SPECT/CT imaging and radiotherapy of tumors.,Biomaterials Science

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Charge-conversional polyethylenimine-entrapped gold nanoparticles with 131I-labeling for enhanced dual mode SPECT/CT imaging and radiotherapy of tumors.
Biomaterials Science ( IF 5.8 ) Pub Date : 2020-06-10 , DOI: 10.1039/d0bm00649a
Jingyi Zhu ₁ , Lingzhou Zhao , Pingping Zhao , Junxing Yang , Jianhui Shi , Jinhua Zhao

Affiliation

Novel theranostic nanosystems demonstrate great potential to achieve timely diagnosis and effective therapy at the same time. However, due to the relatively low accumulation of theranostic nanosystems at the tumor site, the theranostic efficiency is limited. In this study, a novel theranostic nanosystem with a pH-responsive charge conversion property was constructed to improve the cellular uptake towards cancer cells for enhanced single photon emission computed tomography (SPECT)/computed tomography (CT) dual mode imaging and radiotherapy of tumors. In detail, polyethylenimine (PEI) was utilized as a nanoplatform to link with polyethylene glycol (PEG) monomethyl ether with one end of N-hydroxylsuccinimide (mPEG-NHS), PEG with ends of maleimide and succinimidyl valerate (MAL-PEG-SVA), alkoxyphenyl acylsulfonamide (APAS), 3-(4′-hydroxyphenyl)propionic acid-OSu (HPAO), and fluorescein isothiocyanate (FI), successively. The formed functionalized PEI was then utilized to entrap gold nanoparticles, acetylate the remaining amines of PEI and label with radioactive iodine-131 (¹³¹I) to build theranostic nanosystems. The result demonstrated that the theranostic nanosystem has a 3.8 nm Au core and showed excellent colloidal stability. On account of the charge conversion property of APAS, the APAS linked PEI entrapped gold nanoparticles could switch from neutral to positive in a slightly acidic microenvironment, which induced improved cellular uptake. Above all, after ¹³¹I labeling, the generated theranostic nanosystem could achieve enhanced SPECT/CT dual mode imaging and radiotherapy of cancer cells in vitro and a xenograft tumor model in vivo. The constructed APAS-linked PEI nanosystem has great potential to be used as a model for SPECT/CT imaging and radiotherapy of various types of cancer.

中文翻译：

具有131I标记的电荷转化聚乙烯亚胺包裹的金纳米颗粒，用于增强的双模SPECT / CT成像和肿瘤放射疗法。

新型的治疗纳米系统显示出巨大的潜力，可以同时实现及时诊断和有效治疗。然而，由于在肿瘤部位的治疗药物纳米系统的相对较低的积累，治疗药物的效率受到限制。在这项研究中，构建了具有pH响应电荷转换特性的新型治疗疗法纳米系统，以提高细胞对癌细胞的摄取，从而增强单光子发射计算机断层扫描（SPECT）/计算机断层扫描（CT）双模成像和肿瘤的放射疗法。详细地，将聚乙烯亚胺（PEI）用作纳米平台，以与聚乙二醇（PEG）单甲基醚和一个N-羟基琥珀酰亚胺（mPEG-NHS），端基为马来酰亚胺和戊酸琥珀酰亚胺基的PEG（MAL-PEG-SVA），烷氧基苯基酰磺酰胺（APAS），3-（4'-羟基苯基）丙酸-OSu（HPAO）和异硫氰酸荧光素（FI），依次。然后将形成的功能化的PEI用来捕获金纳米颗粒，乙酰化PEI的其余胺，并用放射性碘131（¹³¹ I）标记，以构建诊断学纳米系统。结果表明，治疗纳米系统具有3.8 nm的Au核，并显示出优异的胶体稳定性。由于APAS的电荷转换特性，在微酸性的微环境中，被APAS连接的PEI包裹的金纳米颗粒可以从中性转变为正性，从而改善了细胞的吸收。最重要的是，在¹³¹之后在标记上，生成的治疗纳米系统可以在体外实现增强的SPECT / CT双模成像和癌细胞放射疗法，并在体内实现异种移植肿瘤模型。构建的与APAS相连的PEI纳米系统具有巨大的潜力，可以用作各种癌症的SPECT / CT成像和放射治疗的模型。

更新日期：2020-07-14

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