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Parallelized Wireless Sensing System for Continuous Monitoring of Microtissue Spheroids.
ACS Sensors ( IF 8.2 ) Pub Date : 2020-06-10 , DOI: 10.1021/acssensors.0c00481 Lei Dong 1, 2 , Paolo S Ravaynia 2 , Qing-An Huang 1 , Andreas Hierlemann 2 , Mario M Modena 2
ACS Sensors ( IF 8.2 ) Pub Date : 2020-06-10 , DOI: 10.1021/acssensors.0c00481 Lei Dong 1, 2 , Paolo S Ravaynia 2 , Qing-An Huang 1 , Andreas Hierlemann 2 , Mario M Modena 2
Affiliation
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Currently, the use of electrical readout methods for the investigation of microtissue spheroids in combination with lab automation tools is hindered by the cable connections that are required to interrogate the on-chip-integrated electrodes. To overcome this limitation, we developed a wireless sensor scheme, which can detect the size variation of microtissues during long-term culturing and drug exposure assays. The sensor system includes an interrogation board, which is composed of an inductor–capacitor (LC) readout circuit, and the tissue culture platform with integrated split-ring sensors. The magnetic coupling between the LC circuit and the sensors enables the interrogation of the on-chip sensors without any wire connection to the culture platform. By optimizing the sensor dimensions and the LC resonance frequencies, we were able to avoid cross talk between neighboring sensors. We integrated 12 tissue compartments on a standard microscopy slide with a sensor-to-sensor pitch of 9 mm, which is in accordance with standard 96-well plate dimensions. As a proof-of-concept experiment for the developed system, we monitored continuously and during more than four days the growth inhibition of colon cancer microtissue spheroids that had been exposed to different concentrations of doxorubicin, a chemotherapeutic compound. The stability of the measurements during long-term culturing and the compatibility of the sensor scheme with standard lab equipment offer great potential for automated electrical microtissue spheroid characterization.
中文翻译：
用于连续监测微组织球体的并行无线传感系统。
目前，使用电读出方法与实验室自动化工具相结合来研究微组织球体受到询问片上集成电极所需的电缆连接的阻碍。为了克服这一限制，我们开发了一种无线传感器方案，它可以在长期培养和药物暴露测定过程中检测微组织的尺寸变化。传感器系统包括由电感电容（LC）读出电路组成的询问板和带有集成开口环传感器的组织培养平台。 LC 电路和传感器之间的磁耦合可以询问片上传感器，而无需与培养平台进行任何电线连接。通过优化传感器尺寸和 LC 谐振频率，我们能够避免相邻传感器之间的串扰。我们将 12 个组织隔室集成在标准显微镜载玻片上，传感器间间距为 9 毫米，符合标准 96 孔板尺寸。作为所开发系统的概念验证实验，我们在四天多的时间内连续监测暴露于不同浓度阿霉素（一种化疗化合物）的结肠癌微组织球体的生长抑制。长期培养过程中测量的稳定性以及传感器方案与标准实验室设备的兼容性为自动化电微组织球体表征提供了巨大的潜力。
更新日期：2020-07-24
中文翻译：
用于连续监测微组织球体的并行无线传感系统。
目前，使用电读出方法与实验室自动化工具相结合来研究微组织球体受到询问片上集成电极所需的电缆连接的阻碍。为了克服这一限制，我们开发了一种无线传感器方案，它可以在长期培养和药物暴露测定过程中检测微组织的尺寸变化。传感器系统包括由电感电容（LC）读出电路组成的询问板和带有集成开口环传感器的组织培养平台。 LC 电路和传感器之间的磁耦合可以询问片上传感器，而无需与培养平台进行任何电线连接。通过优化传感器尺寸和 LC 谐振频率，我们能够避免相邻传感器之间的串扰。我们将 12 个组织隔室集成在标准显微镜载玻片上，传感器间间距为 9 毫米，符合标准 96 孔板尺寸。作为所开发系统的概念验证实验，我们在四天多的时间内连续监测暴露于不同浓度阿霉素（一种化疗化合物）的结肠癌微组织球体的生长抑制。长期培养过程中测量的稳定性以及传感器方案与标准实验室设备的兼容性为自动化电微组织球体表征提供了巨大的潜力。
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