Cardiopulmonary dirofilariosis caused by Dirofilaria immitis produces inflammation, blood vessel obstruction and hypoxia, which are required conditions for the beginning of the process of neovascularization. Since D. immitis harbours intracellular symbiotic Wolbachia bacterium, the global understanding of the angiogenic process requires the analysis of the effect of the parasite molecules, but also that of Wolbachia. Canine primary lung microvascular endothelial cells were treated with the recombinant Wolbachia surface protein (rWSP) and the expression of angiogenic factors like Vascular Endothelial Growth Factor-A (VEGF-A), sFlt, membrane Endoglin (mEndoglin) and soluble Endoglin (sEndoglin), as well as the in vitro formation of pseudocapillaries, were measured. The analyses showed a significant increase in the expression of pro-angiogenic VEGF-A and anti-angiogenic sEndoglin, together with a significant decrease in both pro-angiogenic mEndoglin and pseudocapillary formation, compared to untreated controls. Due to the complexity of the angiogenic process and its relationship with other physiological processes like inflammation and fibrinolysis, these results might suggest that rWSP participate in various mechanisms related to each other and its effects might depend either on the balance between them or on the moment of their occurrence.

中文翻译：

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心肺纤丝虫病中的血管生成：Wolbachia 表面蛋白是否具有促血管生成或抗血管生成作用？

引起的心肺纤丝虫病毛丝虫产生炎症、血管阻塞和缺氧，这些是新血管形成过程开始的必要条件。自从D. immitis细胞内共生沃尔巴克氏体细菌，对血管生成过程的全面了解需要分析寄生虫分子的作用，但也需要分析寄生虫分子的作用。沃尔巴克氏体. 重组体处理犬原代肺微血管内皮细胞沃尔巴克氏体表面蛋白 (rWSP) 和血管生成因子如血管内皮生长因子-A (VEGF-A)、sFlt、膜内皮糖蛋白 (mEndoglin) 和可溶性内皮糖蛋白 (sEndoglin) 的表达，以及体外测量假毛细血管的形成。分析显示，与未处理的对照相比，促血管生成 VEGF-A 和抗血管生成 sEndoglin 的表达显着增加，同时促血管生成 mEndoglin 和假毛细血管形成显着减少。由于血管生成过程的复杂性及其与炎症和纤维蛋白溶解等其他生理过程的关系，这些结果可能表明 rWSP 参与了相互关联的各种机制，其作用可能取决于它们之间的平衡或他们的发生。