Engineered PQQ-Dependent Alcohol Dehydrogenase for the Oxidation of 5-(Hydroxymethyl)furoic Acid,ACS Catalysis

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Engineered PQQ-Dependent Alcohol Dehydrogenase for the Oxidation of 5-(Hydroxymethyl)furoic Acid
ACS Catalysis ( IF 11.3 ) Pub Date : 2020-06-09 , DOI: 10.1021/acscatal.0c01789
Matthias Wehrmann ₁ , Eslam M. Elsayed _{2,

3} , Sebastian Köbbing ₄ , Laura Bendz ₁ , Alexander Lepak _{2,

5} , Johannes Schwabe ₂ , Nick Wierckx _{4,

6} , Gert Bange ₂ , Janosch Klebensberger ₁

Affiliation

Furan-2,5-dicarboxylic acid (FDCA) is a bio-based platform chemical with the potential to replace terephthalic acid in the production of polymers. A critical step for enzymatic and whole-cell production of FDCA from 5-(hydroxymethyl)furfural (HMF) is the transformation of 5-(hydroxymethyl)furoic acid (HMFA) into 5-formylfuroic acid (FFA). Here, we establish periplasmic pyrroloquinoline quinone (PQQ)-dependent alcohol dehydrogenases (ADHs) as biocatalytic tools for the oxidation of HMFA, HMF, and 5-formylfurfural (FFF). Further, we identify several amino acid residues including the “lid loop” of the substrate channel as promising targets for future engineering steps toward a fully periplasmic oxidation pathway to FDCA.

中文翻译：

工程化的PQQ依赖性酒精脱氢酶可氧化5-（羟甲基）糠酸

呋喃-2,5-二羧酸（FDCA）是一种生物基平台化学品，有可能在聚合物生产中取代对苯二甲酸。从5-（羟甲基）糠醛（HMF）酶促和全细胞生产FDCA的关键步骤是将5-（羟甲基）糠酸（HMFA）转化为5-甲酰基糠酸（FFA）。在这里，我们建立周质吡咯并喹啉醌（PQQ）依赖的醇脱氢酶（ADHs）作为HMFA，HMF和5-甲酰糠醛（FFF）氧化的生物催化工具。此外，我们确定了一些氨基酸残基，包括底物通道的“闭环”，是未来工程学步骤的有希望的目标，这些步骤是通往FDCA的全周质氧化途径。

更新日期：2020-07-17

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