Reactive Oxygen Species Scavenging and Biodegradable Peptide Hydrogel as 3D Culture Scaffold for Cardiomyocytes,ACS Biomaterials Science & Engineering

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Reactive Oxygen Species Scavenging and Biodegradable Peptide Hydrogel as 3D Culture Scaffold for Cardiomyocytes
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-06-09 , DOI: 10.1021/acsbiomaterials.0c00340
Zhiwei Shen _{1,

2} , Zhen Guo _{1,

2} , Tingyuan Tan _{1,

2} , Jun Hu _{1,

3} , Yi Zhang _{1,

3}

Affiliation

Myocardial ischemia–reperfusion produces a large amount of reactive oxygen species (ROS), which damage the myocardial tissue. Therefore, localized scavenging of ROS from the myocardial tissue would reduce its damage and avoid metabolic abnormalities caused by systemic ROS. In this study, a free radical scavenging and biodegradable supramolecular peptide (ECAFF, named as ECF-5) hydrogel was designed as a culture scaffold for cardiomyocytes. The peptide hydrogel significantly preserved the migration and proliferation of cardiomyocytes and reduced their damage from oxidative stress. In addition, the hydrogel degraded during cell growth, which implies that it may avoid thrombosis of the capillaries in practical use and provide the opportunity for the cells to attach to each other and form a functional tissue. The hydrogel can be used as a 3D culture scaffold for cardiomyocyte culture and allow cardiomyocytes to grow into tissue-like cell spheres. The excellent nature of the ECF-5 hydrogel enables it to have broad applications in the biomedical field in the future.

中文翻译：

活性氧清除和可生物降解的肽水凝胶作为心肌细胞的3D培养支架。

心肌缺血-再灌注会产生大量的活性氧（ROS），从而损害心肌组织。因此，从心肌组织中局部清除ROS将减少其损害并避免由全身性ROS引起的代谢异常。在这项研究中，设计了一种清除自由基和可生物降解的超分子肽（ECAFF，命名为ECF-5）水凝胶作为心肌细胞的培养支架。肽水凝胶可显着保留心肌细胞的迁移和增殖，并减少其因氧化应激而造成的损害。另外，水凝胶在细胞生长过程中降解，这意味着在实际使用中它可以避免毛细血管的血栓形成，并为细胞提供了彼此附着并形成功能组织的机会。该水凝胶可用作心肌细胞培养的3D培养支架，并允许心肌细胞生长成组织样细胞球。ECF-5水凝胶的卓越性能使其在未来的生物医学领域具有广泛的应用。

更新日期：2020-07-13

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