Abstract Histone deacetylases (HDACs) can regulate the progression of various cancers, while their roles in oral cancer cells are not well known. Our present study found that the HDAC1 was over expressed in oral squamous cell carcinoma (OSCC) cells and tissues. Targeted inhibition of HDAC1 via its specific inhibitor PCI24781 or siRNA can inhibit the proliferation of OSCC cells and increase their sensitivity to the chemo-sensitivity such as doxorubicin treatment. HDAC1 can regulate the expression of proliferating cell nuclear antigen (PCNA) via decreasing its mRNA stability. While over expression of PCNA can attenuate HDAC1 inhibition induced suppression of cell proliferation. We checked the expression of various miRNAs which can target the 3′UTR of PCNA. Results showed that HDAC1 can negative regulate the expression of miR-154-5p, inhibitor of miR-154-5p can attenuate PCI24781 treatment decreased PCNA expression and cell proliferation. Collectively, our present study suggested that HDAC1 can promote the growth and progression of OSCC via regulation of miR-154-5p/PCNA signals.

中文翻译：

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组蛋白去乙酰化酶 1 通过 miR-154-5p/PCNA 轴调控口腔癌细胞的恶性程度

摘要 组蛋白去乙酰化酶 (HDAC) 可以调节多种癌症的进展，但它们在口腔癌细胞中的作用尚不清楚。我们目前的研究发现 HDAC1 在口腔鳞状细胞癌 (OSCC) 细胞和组织中过度表达。通过其特异性抑制剂 PCI24781 或 siRNA 靶向抑制 HDAC1 可以抑制 OSCC 细胞的增殖并增加其对化学敏感性如阿霉素治疗的敏感性。HDAC1 可以通过降低其 mRNA 稳定性来调节增殖细胞核抗原 (PCNA) 的表达。而 PCNA 的过度表达可以减弱 HDAC1 抑制诱导的细胞增殖抑制。我们检查了可以靶向 PCNA 3'UTR 的各种 miRNA 的表达。结果表明HDAC1可以负调控miR-154-5p的表达，miR-154-5p抑制剂可以减弱PCI24781处理降低PCNA表达和细胞增殖。总的来说，我们目前的研究表明 HDAC1 可以通过调节 miR-154-5p/PCNA 信号促进 OSCC 的生长和进展。