The endolysin of the Acinetobacter baumannii phage vB_AbaP_D2 shows broad antibacterial activity,Microbial Biotechnology

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The endolysin of the Acinetobacter baumannii phage vB_AbaP_D2 shows broad antibacterial activity
Microbial Biotechnology ( IF 4.8 ) Pub Date : 2020-06-10 , DOI: 10.1111/1751-7915.13594
Yuyu Yuan ₁ , Xiaoyu Li _{1,

2} , Lili Wang _{1,

2} , Gen Li ₁ , Cong Cong ₁ , Ruihua Li ₃ , Huijing Cui ₁ , Bilal Murtaza ₁ , Yongping Xu _{1,

2}

Affiliation

The emergence and rapid spread of multidrug‐resistant bacteria has induced intense research for novel therapeutic approaches. In this study, the Acinetobacter baumannii bacteriophage D2 (vB_AbaP_D2) was isolated, characterized and sequenced. The endolysin of bacteriophage D2, namely Abtn‐4, contains an amphipathic helix and was found to have activity against multidrug‐resistant Gram‐negative strains. By more than 3 log units, A. baumannii were killed by Abtn‐4 (5 µM) in 2 h. In absence of outer membrane permeabilizers, Abtn‐4 exhibited broad antimicrobial activity against several Gram‐positive and Gram‐negative bacteria, such as Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia, Enterococcus and Salmonella. Furthermore, Abtn‐4 had the ability to reduce biofilm formation. Interestingly, Abtn‐4 showed antimicrobial activity against phage‐resistant bacterial mutants. Based on these results, endolysin Abtn‐4 may be a promising candidate therapeutic agent for multidrug‐resistant bacterial infections.

中文翻译：

鲍曼不动杆菌噬菌体vB_AbaP_D2的内溶素表现出广泛的抗菌活性

多药耐药细菌的出现和迅速传播引起了人们对新型治疗方法的深入研究。在这项研究中，鲍曼不动杆菌噬菌体D2（vB_AbaP_D2）进行了分离，鉴定和测序。噬菌体D2的内溶素Abtn-4含有两亲性螺旋，被发现具有抗多药耐药的革兰氏阴性菌株的活性。通过3个对数单位，鲍曼不动杆菌在2小时内被Abtn-4（5 µM）杀死。在没有外膜通透剂的情况下，Abtn-4对几种革兰氏阳性和革兰氏阴性细菌（如金黄色葡萄球菌，铜绿假单胞菌，肺炎克雷伯菌，肠球菌和沙门氏菌。此外，Abtn-4具有减少生物膜形成的能力。有趣的是，Abtn-4对噬菌体耐药细菌突变体表现出抗菌活性。基于这些结果，溶血素Abtn-4可能是多药耐药细菌感染的有前途的候选治疗剂。

更新日期：2020-06-10

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