An Enzyme‐Activable Noncovalent Fluorescent Probe Based on Water Soluble Azobenzene Containing Polymer and AIEgen,Macromolecular Chemistry and Physics

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An Enzyme‐Activable Noncovalent Fluorescent Probe Based on Water Soluble Azobenzene Containing Polymer and AIEgen
Macromolecular Chemistry and Physics ( IF 2.5 ) Pub Date : 2020-06-09 , DOI: 10.1002/macp.202000126
Jiajia Shen ₁ , Tianhao Xue ₁ , Yaning He ₁

Affiliation

Covalent connected fluorescent probe is often effective, but sometimes it is inevitable to adopt complex molecular design and tedious synthesis process. With the advantages of relatively simple operability and widespread generality, noncovalent strategy is gradually considered an ideal choice to solve this dilemma. In this work, an enzyme‐activatable noncovalent fluorescent probe based on water soluble positively charged azobenzene containing poly(ethylene oxide)‐b‐poly[2‐(methacryloyloxy)‐N,N,N‐trimethylethan‐1‐aminium iodide‐co‐(4‐(4‐dimethylamino)phenylazophenyl methacrylate)] (PEG‐b‐PQDMA‐co‐PAZOMA) and negatively charged 3,3′‐(((2‐(4‐(2,2‐dicyano‐1‐phenylvinyl)phenyl)‐2‐phenylethene‐1,1‐diyl)bis(4,1‐phenylene))bis(oxy))bis(propane‐1‐sulfonate) (TPE‐2SO₃⁻) is reported. Due to the fluorescence resonance energy transfer process from aggregates induced emission donor to azobenzene acceptor, this probe is nearly non‐emissive. After the reduction of azo bond by azoreductase under hypoxic condition, the probe emits strong fluorescence with long emission wavelength, realizing efficient off–on property. In addition, the cleavage of the azobenzene do not show influence on the morphology of the nano sized self‐assemblies.

中文翻译：

基于水溶性偶氮苯聚合物和AIEgen的酶活非共价荧光探针

共价连接的荧光探针通常是有效的，但有时不可避免地采用复杂的分子设计和繁琐的合成过程。由于具有相对简单的可操作性和广泛的普遍性的优势，非共价策略逐渐被认为是解决这一难题的理想选择。在这项工作中，酶-可活化的非共价荧光探针基于水溶性正电含有聚偶氮苯（环氧乙烷） - b -聚[2-（甲基丙烯酰氧基）-N，N，N-trimethylethan -1-铵碘化物共- （4-（4-二甲基氨基）苯基偶氮苯基甲基丙烯酸酯）]（PEG- b- PQDMA- co-PAZOMA）和带负电荷的3,3'-（（（（2-（4-（2,2-二氰基-1-苯基乙烯基）苯基）-2-苯基乙烯-1,1-二苯基）双（4,1-苯基）））二（氧基））双（丙烷-1-磺酸酯）（TPE-2 SO ₃^-）被报告。由于从聚集体诱导的发射供体到偶氮苯受体的荧光共振能量转移过程，该探针几乎是非发射性的。在缺氧条件下，偶氮还原酶将偶氮键还原后，探针发出的荧光强度高，发射波长长，可实现有效的导通特性。此外，偶氮苯的裂解对纳米级自组装体的形态没有影响。

更新日期：2020-06-09

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