The present study developed and validated a selective spectrofluorimetric method designed to assay fluvoxamine maleate (FLX). The validated method relied on the condensation reaction between FLX and acetylacetone/formaldehyde using acetate buffer (pH 4.2). The formed fluorescent product was measured at an emission wavelength of 479 nm after excitation at 419 nm. Parameters that influenced the reaction were studied and adjusted accurately. The constructed calibration graph was rectilinear over the range 200–2000 ng ml⁻¹ and the estimated limit of detection was 60 ng ml⁻¹. Two products from an Egyptian market were assayed using the suggested method and the final results agreed with the measurements of the reported method. The selectivity of the proposed approach was evaluated using the standard addition method and results were acceptable and confirmed the reliability of this approach. Finally, directives from the International Council for Harmonisation guidelines were applied to establish the validity of the study.

中文翻译：

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乙酰丙酮用于纯形式和药物制剂的马来酸氟伏沙明的纳米级分析。

本研究开发并验证了一种选择性荧光光谱法，用于测定马来酸氟伏沙明（FLX）。经验证的方法依赖于使用乙酸盐缓冲液（pH 4.2）在FLX与乙酰丙酮/甲醛之间的缩合反应。在419nm激发后，在479nm的发射波长下测量形成的荧光产物。研究并精确调整了影响反应的参数。所构建的校准图在200–2000 ng ml ^-1范围内呈直线，估计检出限为60 ng ml ^-1。使用建议的方法对埃及市场上的两种产品进行了分析，最终结果与报告方法的测量结果一致。使用标准添加方法评估了所提出方法的选择性，结果是可以接受的，并证实了该方法的可靠性。最后，国际协调委员会准则的指令被用于确定研究的有效性。