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Generation of a human induced pluripotent stem cell line (SHUPLi001-A) from PBMCs of a healthy male donor.
Stem Cell Research ( IF 0.8 ) Pub Date : 2020-06-10 , DOI: 10.1016/j.scr.2020.101871
Yijun Cai 1 , Junyi Lin 2 , Kaijun Ma 3 , Meng He 2 , Beixu Li 4
Affiliation  

Human induced pluripotent stem cells (iPSCs) are an ideal model for pathomechanism investigation and drug screening due to their differentiation potency. Here, we generated an iPSC line, SHUPLi001-A, from peripheral blood mononuclear cells (PBMCs) of a healthy 30-year-old male individual using non-integrating Sendai virus. Characterization of SHUPLi001-A was confirmed by the expression of typical pluripotency markers and by the teratoma formation assay to assess differentiation potency in vivo.



中文翻译:


从健康男性捐赠者的 PBMC 中生成人类诱导多能干细胞系 (SHUPLi001-A)。



人类诱导多能干细胞(iPSC)因其分化能力而成为病理机制研究和药物筛选的理想模型。在这里,我们使用非整合仙台病毒从健康 30 岁男性个体的外周血单核细胞 (PBMC) 中生成了 iPSC 系 SHUPLi001-A。 SHUPLi001-A 的表征通过典型多能性标记物的表达和畸胎瘤形成测定来证实,以评估体内分化能力。

更新日期:2020-06-10
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