The use of a metallic adhesion layer between plasmonic-active nanostructures and a solid support is known to dampen the plasmonic response. To overcome this problem, organic adhesion layers have been introduced, which in turn can undermine the stability of the film. Moreover, both types of layers limit the regeneration of the nanostructures for multiple uses. Here we report a quick and simple approach to prepare intermediate adhesion layer-free binding of nanostructured films of gold on silicon wafers. The approach involves scratching and etching of the silicon wafer before sputter coating with a thin layer of Au. The plasmonic-active nanostructures were then prepared on this thin Au film using electrochemical deposition. As-prepared plasmonic-active nanostructured thin films of gold (PANTF-Au) are easy to handle, physically robust, and can be regenerated. The bulk refractive index sensitivity of PANTF-Au is 150 nm/RIU with the figure of merit 1.4, and with a plasmonic field-decay length of 27 nm. We further used these thin films to study interactions between lectin and glycoprotein inside a flow cell as well as on a microplate made of PANTF-Au. The PANTF-Au can be easily integrated with electrochemical devices and microfluidics, which can help to pave the way toward the development of ideal optical-electrochemical point-of-care biosensors.

已知在等离子体活性纳米结构和固体支撑物之间使用金属粘附层可以抑制等离子体响应。为了克服这个问题，引入了有机粘合层，但这反过来又会破坏薄膜的稳定性。此外，两种类型的层都限制了纳米结构的多种用途的再生。在这里，我们报告了一种快速而简单的方法来制备硅晶圆上金纳米结构薄膜的无中间粘附层的结合。该方法包括在溅射镀上一层薄薄的金之前对硅晶片进行刮擦和蚀刻。然后利用电化学沉积在金薄膜上制备等离子体活性纳米结构。所制备的等离子体活性纳米结构金薄膜 (PANTF-Au) 易于处理、物理坚固且可以再生。 PANTF-Au 的体折射率灵敏度为 150 nm/RIU，品质因数为 1.4，等离子体场衰减长度为 27 nm。我们进一步使用这些薄膜来研究流动池内以及由 PANTF-Au 制成的微孔板上的凝集素和糖蛋白之间的相互作用。 PANTF-Au 可以轻松地与电化学设备和微流体集成，这有助于为理想的光学电化学即时生物传感器的开发铺平道路。