Introduction

Polyglycolic acid (PGA) nerve conduits, an artificial biodegradable nerve regeneration-inducing tube currently used in clinical practice, are effective in regenerating peripheral nerves. Dedifferentiated fat (DFAT) cells differentiate into various cells including adipocytes, osteoblasts, chondrocytes, skeletal muscle cells, and myofibroblasts, when cultured in appropriate differentiation-inducing conditioned culture medium. This study made a hybrid artificial nerve conduit by filling a PGA conduit with DFAT cells, applied the conduit to a rat facial nerve defect model, and investigated the facial nerve regenerative ability of the conduit.

Methods

Under inhalational anesthesia, the buccal branch of the facial nerve in Lewis rats was exposed, and a 7-mm nerve defect was created. PGA nerve conduits were filled with DFAT cells, which were prepared from rat subcutaneous adipose tissue with type I collagen as a scaffold, and then grafted into the nerve defect sites in rats with a microscope (DFAT group) (n = 10). In other rats, PGA artificial nerve conduits alone were similarly grafted into the nerve defect sites (the control group) (n = 10). Reinnervation was confirmed at 13 weeks postoperatively by a retrograde tracer, followed by histological and physiological comparative studies.

Results

The mean number of myelinated fibers was significantly higher in DFAT group (1606 ± 806) than in the control group (543 ± 478). Myelin thickness was also significantly lager in DFAT group (0.67 ± 0.02 μm) than in the control group (0.43 ± 0.01 μm). Although no significant difference was found in the amplitude of compound muscle action potential (CMAP) between DFAT group (2.84 ± 2.47 mV) and the control group (0.88 ± 0.56 mV), whisker motion was lager in DFAT group (9.22° ± 0.65°) than in the control group (1.9° ± 0.84°).

Conclusions

DFAT cell-filled PGA conduits were found to promote nerve regeneration in an experimental rat facial nerve defect model.

中文翻译：

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“聚乙醇酸-胶原神经导管中的去分化脂肪细胞促进大鼠面部神经再生”[Regen Ther 11 (2019) 240-248] 的勘误表。

介绍

聚乙醇酸（PGA）神经导管是目前临床上使用的人工可生物降解神经再生诱导管，可有效再生周围神经。当在适当的诱导分化的条件培养基中培养时，去分化脂肪 (DFAT) 细胞分化成各种细胞，包括脂肪细胞、成骨细胞、软骨细胞、骨骼肌细胞和肌成纤维细胞。本研究通过用DFAT细胞填充PGA导管制作混合人工神经导管，将该导管应用于大鼠面神经缺损模型，并研究该导管的面神经再生能力。

方法

在吸入麻醉下，Lewis 大鼠面神经颊支暴露，形成 7 mm 神经缺损。PGA神经导管内填充DFAT细胞，以大鼠皮下脂肪组织为支架，I型胶原蛋白为支架，然后在显微镜下移植到大鼠神经缺损部位（DFAT组）（n = 10）。在其他大鼠中，仅将 PGA 人工神经导管类似地移植到神经缺损部位（对照组）（n = 10）。术后 13 周通过逆行示踪剂确认神经再生，然后进行组织学和生理学比较研究。

结果

DFAT 组的平均有髓纤维数 (1606 ± 806) 明显高于对照组 (543 ± 478)。DFAT组的髓鞘厚度（0.67±0.02μm）也明显大于对照组（0.43±0.01μm）。虽然DFAT组（2.84±2.47 mV）和对照组（0.88±0.56 mV）的复合肌肉动作电位（CMAP）幅度没有显着差异，但DFAT组的晶须运动更大（9.22°±0.65°） ) 比对照组 (1.9° ± 0.84°)。

结论

在实验性大鼠面部神经缺损模型中，发现充满 DFAT 细胞的 PGA 导管可促进神经再生。