Two new C₁₉-diterpenoid alkaloids, smirnotine A (1) and smirnotine B (2), together with five known diterpenoid alkaloids (3-7) were isolated from the aerial part of Aconitum smirnovii Steinb. The structures of new compounds were established on the basis of spectroscopic analyses, including HR-ESI-MS, 1D and 2D NMR spectroscopic data, while the absolute configuration of 1 was determined by quantum ECD calculation. The antiarrhythmic, cytotoxic and antimicrobial activities of these diterpenoid alkaloids were also evaluated. Compound 1 showed preventive effect of arrhythmia on arrhythmia model of anesthetized mice induced by aconitine.

从乌头乌头的空中分离出两个新的C _19-二萜生物碱，smirnotine A（1）和smirnotine B（2）以及五种已知的二萜生物碱（3-7）。新化合物的结构是在光谱分析的基础上建立的，包括HR-ESI-MS，1D和2D NMR光谱数据，而1的绝对构型是通过量子ECD计算确定的。还评估了这些二萜类生物碱的抗心律失常，细胞毒性和抗菌活性。化合物1显示出心律不齐对乌头碱诱导的麻醉小鼠的心律失常模型的预防作用。