Farnesene is a typical sesquiterpene with applications as fragrance, flavor and precursor for the synthesis of vitamin E/K1. In this study, a series of strategies were employed to facilitate α-farnesene accumulation in Yarrowia lipolytica. Among them, the promoter optimization of OptFSLERG20, Sc-tHMG1 and IDI resulted in more than 62 % increase in α-farnesene production. Together with the overexpression of Yl-HMGR and ERG19, α-farnesene content was significantly improved by more than 3.5 times. The best metabolic engineered strain obtained was therefore used for a uniform design in shake flasks to determine the optimal medium compositions. Furthermore, a maximum α-farnesene production of approximately 2.57 g/L (34 mg/g DCW) was obtained in fed-batch fermentation where glycerol was supplemented as the feeding carbon source when initial glucose was depleted. This study has laid a good foundation for the development of Y. lipolytica as a promising chassis microbial cell for heterologous biosynthesis of α-farnesene and other sesquiterpenes.

法呢烯是一种典型的倍半萜烯，可用作香料，香料和维生素E / K1合成的前体。在这项研究中，采用了一系列策略来促进解脂耶氏酵母中α-法尼烯的积累。其中，通过优化OptFSLERG20，Sc-tHMG1和IDI的启动子，α-法呢烯产量提高了62％以上。连同Yl-HMGR和ERG19的过表达，α-法尼烯含量显着提高了3.5倍以上。因此，将获得的最佳代谢工程菌株用于摇瓶中的均匀设计，以确定最佳培养基组成。此外，在补料分批发酵中获得了大约2.57 g / L（34 mg / g DCW）的最大α-法呢烯产量，当初始葡萄糖耗尽时，其中补充了甘油作为补料碳源。该研究为解脂耶氏酵母作为α-法呢烯和其他倍半萜的异源生物合成的有希望的底盘微生物细胞的发展奠定了良好的基础。