Resveratrol (Resv) was tested to assess its effects on buck semen freezability. Ejaculates of 4 bucks were collected, washed and diluted in a commercial extender at 30 °C. Extended semen was divided into 4 aliquots supplemented with increasing concentrations of Resv: 0 μM (control); 10 μM; 25 μM and 50 μM. Aliquots were cooled to 4 °C in 5h and frozen in LN2. Thawing was performed at 37 °C for 30 s. At the 3 stages of the experiment (30 °C, 4 °C, thawing), motility (CASA), osmotic resistance (Hos test) and integrity of cytoplasm and acrosome membranes (PI/PSA staining) were assessed. Moreover, in thawed samples, the oxidative status (MDA assay) and early apoptosis (DNA fragmentation by TUNEL assay) were evaluated. Resveratrol supplementation did not affect most of the motility parameters analysed, except for total motility, ALH (lateral head displacement) and velocity distribution (P < 0.05). Functional and morphological integrity of membranes was not affected at any stage of the experiment (P > 0.05). In thawed spermatozoa, the oxidative status was not preserved by Resv (P > 0.05) while early apoptosis, was significantly decreased in the 50 μM Resv group (P < 0.05). Resveratrol did not improve buck semen freezability; the observed effects on motility and DNA were not dose dependent and not mediated by a potential anti-oxidant activity.

中文翻译：

---

白藜芦醇补充剂和雄鹿精液的冷冻保存

测试白藜芦醇 (Resv) 以评估其对雄鹿精液可冷冻性的影响。收集 4 美元的精液，在 30 °C 下用商用稀释剂清洗和稀释。延长的精液被分成 4 等份，并补充有不断增加的 Resv 浓度：0 μM（对照）；10微米；25 μM 和 50 μM。等分试样在 5 小时内冷却至 4 °C，并在 LN2 中冷冻。解冻在 37°C 下进行 30 秒。在实验的 3 个阶段（30°C、4°C、解冻），评估了运动性（CASA）、渗透阻力（Hos 测试）以及细胞质和顶体膜的完整性（PI/PSA 染色）。此外，在解冻的样品中，评估了氧化状态（MDA 测定）和早期细胞凋亡（通过 TUNEL 测定的 DNA 片段化）。补充白藜芦醇不影响分析的大多数运动参数，除了总运动，ALH（横向头部位移）和速度分布（P < 0.05）。膜的功能和形态完整性在实验的任何阶段都没有受到影响（P > 0.05）。在解冻的精子中，Resv 未保留氧化状态（P > 0.05），而早期凋亡在 50 μM Resv 组中显着降低（P < 0.05）。白藜芦醇没有改善雄鹿精液的可冷冻性；观察到的对运动性和 DNA 的影响不是剂量依赖性的，也不是由潜在的抗氧化活性介导的。50 μM Resv 组显着降低（P < 0.05）。白藜芦醇没有改善雄鹿精液的可冷冻性；观察到的对运动性和 DNA 的影响不是剂量依赖性的，也不是由潜在的抗氧化活性介导的。50 μM Resv 组显着降低（P < 0.05）。白藜芦醇没有改善雄鹿精液的可冷冻性；观察到的对运动性和 DNA 的影响不是剂量依赖性的，也不是由潜在的抗氧化活性介导的。