A miniature biosensing platform based on MgO-based nanoparticle doped optical fiber was developed for the biomolecule detection. The technology used a single mode fiber with MgO-based nanoparticles doped core. The detection was based on collecting the Rayleigh backscattering signatures with increased gain upon the etching of the fiber 1–2 mm away from the tip. The shift from the backscattered signal with the maximum value of the cross-correlation was used to report the results. The sensor exhibited a sensitivity range from 0.75 nm/refractive index unit up to 19.63 nm/refractive index unit for a refractive index range from 1.3329 up to 1.37649. The deposition of the thin gold layer increased the overall sensitivity of the biosensor by 3.7 times for the etched part of the fiber with diameter 8–9 μm. The proposed biosensor was tested for the detection of thrombin molecule concentrations ranging from 0.625 μg/ml to 20 μg/ml. Thiol modified DNA specific aptamers were used to functionalize the gold coated surface of the fiber for the detection. The sensor showed detectable sensitivity and specificity as compared to the other control proteins. The proposed biosensing platform could be multiplexed and can be used in vivo for the detection in clinical settings due to its miniature size, biocompatibility of silica glass and reflector less set up.

开发了基于MgO基纳米粒子掺杂光纤的微型生物传感平台，用于生物分子检测。该技术使用了具有MgO基纳米颗粒掺杂纤芯的单模光纤。该检测是基于在蚀刻距离尖端1-2 mm的光纤时以增加的增益收集瑞利反向散射特征。使用背散射信号与互相关最大值的偏移来报告结果。该传感器的灵敏度范围为0.75 nm /折射率单位至19.63 nm /折射率单位，折射率范围为1.3329至1.37649。薄金层的沉积使直径8–9μm的纤维蚀刻部分的生物传感器的整体灵敏度提高了3.7倍。测试了所提议的生物传感器的凝血酶分子浓度，范围从0.625μg/ ml到20μg/ ml。硫醇修饰的DNA特异性适体用于功能化纤维的金涂层表面以进行检测。与其他对照蛋白相比，该传感器显示出可检测的灵敏度和特异性。拟议中的生物传感平台可以多路复用并可以使用由于其尺寸小，二氧化硅玻璃和反射镜的生物相容性较小，因此可用于临床环境中的体内检测。