Acta Histochemica ( IF 2.3 ) Pub Date : 2020-06-10 , DOI: 10.1016/j.acthis.2020.151569 Kei-Ichiro Uemura 1 , Tokumasa Hayashi 2 , Tasuku Hiroshige 2 , Kosuke Ueda 2 , Keisuke Ohta 3 , Tomonoshin Kanazawa 3 , Shingo Hirashima 3 , Makoto Nakiri 2 , Tsukasa Igawa 2 , Kei-Ichiro Nakamura 3
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Purpose
The fate of subcutaneously transplanted urogenital sinus (UGS) and seminal vesicle (SV) was investigated in the present study.
Materials and Methods
Fetal UGS and SV extracted from 20-embryonic-day-old male normal and GFP transgenic rats were subcutaneously transplanted into 7-week-old male immunologically inhibited rats. The transplants were then examined at 2, 4, 8, and 16 weeks after transplantation. We analyzed the survival ratio, weight, and histopathology as well as the immunohistochemical characteristics of the transplanted tissues. For control experiments, 2-, 4-, 8-, and 16-week-old normal male rats were used.
Results
Almost all of the transplanted tissues survived under the skin, and the tissue weights increased over time after transplantation. The histopathological characteristics and immunohistochemical staining pattern with certain antibodies of the transplanted tissues were similar to those of normal adult rat prostate and seminal vesicle. The transplanted GFP transgenic tissues demonstrated spontaneous growth and organ formation under the skin, showing distribution and movement of transplanted cells and tissues.
Conclusion
Subcutaneously transplanted fetal UGS and SV were able to develop into mature adult organs.
中文翻译：
胎儿大鼠泌尿生殖窦和精囊的异位皮下移植可促进器官的生长和形成。
目的
在本研究中，研究了皮下移植的泌尿生殖窦（UGS）和精囊（SV）的命运。
材料和方法
将从20个胚胎日大的雄性正常人和GFP转基因大鼠中提取的胎儿UGS和SV皮下移植到7周大的受到免疫抑制的雄性大鼠中。然后在移植后第2、4、8和16周检查移植物。我们分析了存活率，体重，组织病理学以及移植组织的免疫组织化学特征。对于对照实验，使用2、4、8、16周龄的正常雄性大鼠。
结果
几乎所有移植的组织都在皮肤下存活，并且移植后组织的重量随着时间的推移而增加。移植组织中某些抗体的组织病理学特征和免疫组化染色模式与正常成年大鼠前列腺和精囊的相似。移植的GFP转基因组织在皮肤下显示出自发生长和器官形成，显示出移植的细胞和组织的分布和运动。
结论
皮下移植的胎儿UGS和SV能够发育成成熟的成人器官。
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