Abstract

Sea buckthorn (Hippophae rhamnoides L.) is a perennial dioecious shrub or tree whose juvenile period lasts from 3 to 5 years. Sea buckthorn grows in many European and Asian countries and is widely used in cosmetology, biotechnology, pharmacology, and agriculture. This crop is a source of valuable vitamins, minerals, carotenoids, and flavonoids. Its phenotypic description does not provide accurate characteristics of a taxon at the species, subspecies, variety, and population levels; therefore, one should use molecular analysis. For this study, a collection of sea buckthorn samples of different ecological and geographical origin, which consisted from herbarium samples and samples harvested in natural populations, was prepared. ISSR markers efficiently revealing genotypic difference between the samples were selected, and binary matrices were created for the further dendrogram construction. The performed analysis revealed a high level of intraspecific polymorphism and showed that samples of a similar ecological and geographical origin tended to group into clusters and were located at different genetic distance. The results of the study can be used to evaluate the diversity of forms of this crop, relations between them, their ecological and geographical origin, and the evolution of certain taxa. These results may also be used for a genetic passportization and identification of varieties prior to them reaching the generative period and for the pair selection for crossing.

中文翻译：

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ISSR标记在不同来源沙棘（Hippophae rhamnoides L.）多态性分析中的应用

摘要

沙棘（沙棘）L.）是多年生雌雄异株的灌木或乔木，幼年期为3至5年。沙棘生长在许多欧洲和亚洲国家，并广泛用于美容，生物技术，药理学和农业。该作物是宝贵的维生素，矿物质，类胡萝卜素和类黄酮的来源。它的表型描述不能提供物种，亚种，品种和种群水平的分类单元的准确特征。因此，应该使用分子分析。为了进行这项研究，准备了一系列具有不同生态和地理起源的沙棘样品，其中包括植物标本室样品和自然种群中采集的样品。选择能有效揭示样品之间基因型差异的ISSR标记，并创建了二进制矩阵以用于进一步的树状图构造。进行的分析显示出高水平的种内多态性，并显示出具有相似生态和地理起源的样本倾向于成簇，并且位于不同的遗传距离。研究结果可用于评估这种作物形式的多样性，它们之间的关系，其生态和地理起源以及某些分类群的演变。这些结果还可以用于遗传护照鉴定和变种进入成年期之前的鉴定，以及用于杂交的配对选择。进行的分析显示出高水平的种内多态性，并显示出具有相似生态和地理起源的样本倾向于成簇，并且位于不同的遗传距离。研究结果可用于评估这种作物形态的多样性，它们之间的关系，其生态和地理起源以及某些分类群的演变。这些结果还可以用于遗传护照鉴定和变种进入成年期之前的鉴定，以及用于杂交的配对选择。进行的分析显示出高水平的种内多态性，并显示出具有相似生态和地理起源的样本倾向于成簇，并且位于不同的遗传距离。研究结果可用于评估这种作物形态的多样性，它们之间的关系，其生态和地理起源以及某些分类群的演变。这些结果还可以用于遗传护照鉴定和变种进入成年期之前的鉴定，以及用于杂交的配对选择。