We report a case of severe congenital toxoplasmosis that involved an atypical T. gondii genotype in a newborn baby from Alagoas state in Northeastern Brazil. A pregnant woman presented IgM and IgG anti-T. gondii antibodies, as detected by the chemiluminescence immunoassay on the second trimester of pregnancy. A mouse bioassay was performed using umbilical cord blood and one isolate was obtained. The isolate was designated TgCTBrAL1 and genetic characterization revealed genotype ToxoDB #162. Genotype results of the rhoptry genes, ROP5 and ROP18, could predict the high virulence of the isolate in mice, which was confirmed by an in vivo virulence assay. This is the first report of generating a T. gondii isolate from a newborn baby with congenital toxoplasmosis in Northeastern Brazil.

我们报告了一个严重的先天性弓形虫病病例，该病例涉及来自巴西东北部阿拉戈斯州的新生婴儿中的非典型弓形虫基因型。一名孕妇在妊娠中期提供了IgM和IgG抗弓形虫抗体，通过化学发光免疫分析法检测到。使用脐带血进行小鼠生物测定，获得一种分离物。该分离株命名为TgCTBrAL1，遗传特征显示基因型ToxoDB＃162。rhoptry基因ROP5和ROP18的基因型结果可以预测分离株在小鼠中的高毒力，这一点已通过体内毒力试验得以证实。这是产生弓形虫的第一份报告 分离自巴西东北部先天性弓形虫病的新生儿。