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Population connectivity and genetic structure of Asian green mussel, Perna viridis along Indian waters assessed using mitochondrial markers.
Molecular Biology Reports ( IF 2.6 ) Pub Date : 2020-06-10 , DOI: 10.1007/s11033-020-05575-4
P R Divya 1 , P M Elizabeth Jency 1 , Linu Joy 1 , A Kathirvelpandian 1 , Rajeev Kumar Singh 2 , V S Basheer 1
Affiliation  

Perna viridis (Linnaeus, 1758), the Asian green mussel, belonging to the family Mytilidae is widely distributed along the Indian coast. The species is majorly found in southeastern countries and is considered an ideal candidate for aquaculture due to its high nutritional value and growth rate. Obtaining their genetic information is essential for their sustainable capture-based production. In the present study, genetic variation, population structure, and demographic processes of the populations across the distribution of this species were assessed using the mitochondrial DNA ATPase6 and cytb gene. In total, we selected 170 samples from five localities across the Indian subcontinent including Andaman Sea. Sequence analysis of partial cytb (885 bp) and ATPase6 (714 bp) genes revealed 45 and 58 haplotypes, respectively. The significant coefficient of genetic differentiation (FST: 0.255 for cytb and 0.252 for ATPase6) and analyses of molecular variance indicated three varieties of stocks, namely Arabian Sea, Bay of Bengal, and Andaman Sea. All the populations showed low nucleotide diversity, suggesting severe historical bottleneck events and high haplotype diversity, indicating population expansion. The genetic variation and demographic process reported in this study will form the baseline information for framing policies, which can be adopted while planning stock specific ranching and relaying programmes in the Indian subcontinent with view to enhance and manage the fishery.



中文翻译:

使用线粒体标记评估了印度洋沿亚洲水域的亚洲绿贻贝(Perna viridis)的种群连通性和遗传结构。

绿蔷薇(Linnaeus,1758年),属于贻贝科的亚洲绿色贻贝,在印度沿岸广泛分布。该物种主要在东南部国家发现,由于其高营养价值和高增长率,被认为是水产养殖的理想人选。获得其遗传信息对于其可持续的基于捕捞的生产至关重要。在本研究中,使用线粒体DNA ATPase6和cytb基因评估了该物种分布的种群的遗传变异,种群结构和人口统计学过程。总共,我们从包括安达曼海在内的印度次大陆的五个地方中选择了170个样本。部分cytb(885 bp)和ATPase6(714 bp)基因的序列分析分别显示45和58个单倍型。F ST:对于cytb为0.255,对于ATPase6为0.252),并且通过分子差异分析显示了三种种群,即阿拉伯海,孟加拉湾和安达曼海。所有种群均显示出低核苷酸多样性,表明存在严重的历史瓶颈事件和高单倍型多样性,表明种群正在扩大。这项研究报告的遗传变异和人口统计过程将构成框架政策的基准信息,可在印度次大陆计划针对特定种群的放牧和中转计划时采用这些信息,以增强和管理渔业。

更新日期:2020-06-10
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