A mixed culture of bioleaching microorganisms was enriched. Then the mixed culture was preserved by liquid nitrogen cryopreservation for 6 months and 12 months, respectively, using PEG-2000 as the protective agent. The chalcopyrite leaching ability, activity and diversity of the mixed culture before and after preservation were compared. The results showed that the copper extraction rate was 95.7% in chalcopyrite bioleaching within 20 d by the original culture. After cryopreservation for 6 months and 12 months, the copper extraction rate of the mixed culture was 94.9% within 25 d and 93.6% within 35 d, respectively. The cell viability achieved 87% and 41% after being preserved for 6 months and 12 months, respectively. Furthermore, the ecology analysis identified Acidithiobacillus ferrooxidans, Acidithiobacillus caldus, Sulfobacillus thermotolerans and Pseudomonas aeruginosa in the original mixed culture. After cryopreservation for 12 months, the composition of community changed, but the predominant microorganisms still existed.

丰富了生物浸出微生物的混合培养物。然后，使用PEG-2000作为保护剂，通过液氮冷冻保存将混合培养物分别保存6个月和12个月。比较了保存前后黄铜矿的黄铜矿浸出能力，活性和多样性。结果表明，原始培养20 d内黄铜矿生物浸出铜的提取率为95.7％。冷冻保存6个月和12个月后，混合培养物在25 d内的铜提取率分别为94.9％和35 d内的93.6％。保存六个月和十二个月后，细胞活力分别达到87％和41％。此外，生态学分析确定了酸性氧化铁硫杆菌，原始混合培养物中的酸性嗜酸杆菌，耐高温硫杆菌和铜绿假单胞菌。冷冻保存12个月后，群落组成发生了变化，但主要微生物仍然存在。