Leptospirillum ferriphilum YSK was added to a native consortium of bioleaching bacteria including Acidithiobacillus caldus, A. thiooxidans, A. ferrooxidans, Sulfobacillus thermosulfidooxidans, Acidiphilium spp., and Ferroplasma thermophilum cultured in modified 9K medium containing 0.5% (W/V) pyrite. The bioleaching efficiency markedly increased. Changes in community structure and gene expression were monitored with real-time PCR and functional gene arrays. Dynamic changes that varied in different populations in the consortium occurred after the addition of L. ferriphilum YSK, with growth of A. caldus S1, A. thiooxidans A01, Acidiphillum spp. DX1-1 promoted the growth of Ferroplasma L1, inhibited that of S. thermosulfidooxidans ST, and exerted little effect on that of A. ferrooxidans CMS. Genes encoding ADP heptose, phosphoheptose isomerase, glycosyltransferase, biotin carboxylase, and protoheme ferrolyase from L. ferriphilum, acetyl-CoA carboxylase from Acidiphillum spp., and doxD from A. caldus were up-regulated in 0–20 h. Genes encoding lipid A disaccharide synthase LpxB, glycosyl transferase, and ADP heptose synthase from A. ferrooxidans were up-regulated in 0–8 h and then down-regulated in 8–20 h. Genes encoding ferredoxin oxidoreductase from Ferroplasma sp. were up-regulated in 0–4 h, down-regulated in 4–16 h, and again up-regulated in 16–20 h. CbbS from A. ferrooxidans was down-regulated in 0–20 h.

钩端螺旋体嗜铁钩端螺旋菌YSK加入浸矿细菌包括本地财团嗜酸喜温，A.杆菌，A.杆菌，硫化杆菌thermosulfidooxidans，Acidiphilium属。，以及在改良的9K培养基（含有0.5％（W / V）黄铁矿）中培养的嗜热亚铁。生物浸出效率显着提高。社区结构和基因表达的变化通过实时PCR和功能基因阵列进行监测。在添加了L. ferriphilum YSK之后，财团中不同种群的动态变化发生了，随着A. caldus S1，A. thiooxidans A01，Acidiphillum spp。DX1-1促进生长铁原体L1，抑制时的S. thermosulfidooxidans ST，和施加在其的影响不大A.杆菌CMS。编码ADP庚糖，phosphoheptose异构酶，糖基转移酶，生物素羧化酶，和从正铁血红素ferrolyase基因L.嗜铁钩端螺旋菌，乙酰辅酶A羧化酶从Acidiphillum属和doxD从A.喜温表达上调在0-20小时。编码脂质A二糖合酶LpxB，糖基转移酶，和ADP基因庚糖从合酶A.杆菌在0-8小时内上调，然后在8-20小时内下调。来自Ferroplasma sp。的编码铁氧还蛋白氧化还原酶的基因。在0–4小时内上调，在4–16小时内下调，然后在16-20小时内再次上调。央行票据从A.杆菌在0-20 h的下调。