The aims of this study were to estimate seasonal spore availability in the carrageenophyte Gigartina skottsbergii from the Patagonian Atlantic coast, evaluate the effect of induction methods and culture conditions on spore release, and assess spore settlement on different substrates. Fertile fronds were collected in southern Argentina. The density and size of reproductive structures and spore output were registered seasonally. Spore release was evaluated using different induction methods and conditions. Finally, different natural substrates were assessed for spore settlement. Spore availability is restricted to winter and spring. Cystocarps and tetrasporangial sori reached maturity towards winter, and spores were released in large amounts during winter and spring, with higher tetraspore than carpospore release. Spontaneous discharge would appear to be a more advisable induction method than desiccation and high salinity. Temperature and photoperiod did not have a significant effect on total spore output. After 2 weeks of incubation, no differences were found among spore densities on glass, pebbles, clams, or mussels. Despite the low survival rate registered on all substrates, spores germinated and developed healthily. This is the first study to evaluate carpospore and tetraspore availability throughout the year and the viability of in vitro spore culture of G. skottsbergii from the South American Atlantic coast.

这项研究的目的是估计角叉菜藻Gigartina skottsbergii中的季节性孢子可用性来自巴塔哥尼亚大西洋沿岸，评估诱导方法和培养条件对孢子释放的影响，并评估孢子在不同基质上的沉降。在阿根廷南部收集了肥沃的叶子。繁殖结构的密度和大小以及孢子的产量按季节记录。使用不同的诱导方法和条件评估孢子释放。最后，评估不同的自然底物的孢子沉降。孢子的供应仅限于冬季和春季。到冬季，囊果和四孢子囊菌就已成熟，在冬季和春季，孢子大量释放，四孢子的释放量高于鲤鱼的释放量。自发放电比干燥和高盐度似乎是更可取的诱导方法。温度和光周期对总孢子产量没有显着影响。孵育2周后，在玻璃，鹅卵石，蛤或贻贝上的孢子密度之间没有发现差异。尽管在所有底物上的存活率都很低，但孢子仍能发芽并健康地发育。这是第一项评估全年孢子和四孢子可用性以及体外培养孢子的可行性的研究。来自南美大西洋沿岸的G. skottsbergii。