The search for promising yeasts that surpass the fermentative capacity of commercial strains, such as Saccharomyces cerevisiae CAT-1, is of great importance for industrial ethanol processes in the world. Two yeasts, Pichia kudriavzevii BB2 and Saccharomyces cerevisiae BB9, were evaluated in comparison to the industrial yeast S. cerevisiae CAT-1. The objective was to evaluate the performance profile of the three studied strains in terms of growth, substrate consumption, and metabolite formation, aiming to determine their behaviour in different media and pH conditions. The results showed that under cultivation conditions simulating the medium used in the industrial process (must at 22° Brix at pH 3.0) the highest ethanol productivity was 0.41 g L⁻¹ h⁻¹ for S. cerevisiae CAT-1, compared to 0.11 g L⁻¹ h⁻¹ and 0.16 g L⁻¹ h⁻¹ for P. kudriavzevii and S. cerevisiae BB2, respectively. S. cerevisiae CAT-1 produced three times more ethanol in must at pH 3.0 (28.30 g L⁻¹) and in mineral medium at pH 3.0 (29.17 g L⁻¹) and 5.0 (30.70 g L⁻¹) when compared to the value obtained in sugarcane must pH 3.0 (9.89 g L⁻¹). It was concluded that S. cerevisiae CAT-1 was not limited by the variation in pH in the mineral medium due to its nutritional composition, guaranteeing better performance of the yeast even in the presence of stressors. Only S. cerevisiae CAT-1 expressed he constitutive invertase enzyme, which is responsible for hydrolysing the sucrose contained in the must.

寻找超越商业菌株发酵能力的有前途的酵母，例如酿酒酵母CAT-1，对于世界上的工业乙醇工艺具有重要意义。与工业酵母S. cerevisiae相比，对两种酵母Pichia kudriavzevii BB2 和Saccharomyces cerevisiae BB9 进行了评估CAT-1。目的是评估三个研究菌株在生长、底物消耗和代谢物形成方面的性能特征，旨在确定它们在不同介质和 pH 条件下的行为。结果表明，培养条件模拟（在pH3.0必须在22°白利糖度）在工业过程中使用的培养基下的最高乙醇产为0.41克L- ^-1  ħ ^-1为酿酒酵母CAT-1，相对于0.11克L ^-1 h ^-1和 0.16 g L ^-1 h ^{-1 分别}用于P. kudriavzevii和S. cerevisiae BB2。酿酒酵母CAT-1 在 pH 3.0 (28.30 g L ^-1 ) 的葡萄汁和 pH 3.0 (29.17 g L ^-1 ) 和 5.0 (30.70 g L ^-1 ) 的矿物培养基中产生的乙醇是在甘蔗的 pH 值必须为 3.0 (9.89 g L ^-1 )。得出的结论是，酿酒酵母CAT-1 由于其营养成分而不受矿物培养基中 pH 变化的限制，即使在存在压力因素的情况下也能保证酵母的更好性能。只有酿酒酵母CAT-1 表达组成型转化酶，该酶负责水解葡萄汁中所含的蔗糖。