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Mechanical and biological properties of polycaprolactone/fibrin nanocomposite adhesive produced by electrospinning method
Bulletin of Materials Science ( IF 1.9 ) Pub Date : 2020-06-09 , DOI: 10.1007/s12034-020-02111-9
Bahare Hushmand Shahrifi , Majid Mohammadi , Mehdi Manoochehri , Amir Atashi

Biopolymer nanocomposites exhibit an important role in regulating cell function in tissue engineering applications. Rapid degradation and good biological activities of these nanocomposites make them suitable for use in orthopaedic and medical applications. In this study, a polycaprolactone (PCL) scaffold and fibrin glue were used as a matrix and reinforcement, respectively. PCL/fibrin nanocomposites with different aspect ratios were synthesized and used as a bioactive adhesive. The tensile strength of PCL/fibrin was measured using a tensile machine-equipped polymer load cell. Microstructural analysis of PCL and PCL/fibrin nanocomposites was investigated by field emission scanning electron microscopy. Microstructure results showed suitable porosity with proper distribution and size in the PCL–fibrin nanocomposites with a 1/10 aspect ratio. The results of wettability showed that fibrin as reinforcement in PCL decreased the contact angle and improved hydrophilic properties. The adhesion properties of PCL and PCL/fibrin scaffolds were determined by the $$4^\prime $$ 4 ′ , 6-diamidino-2-phenylindole test. Mesenchymal stem cells were cultured on the PCL and PCL/fibrin scaffolds. The results showed more and better adhesion of cells on the PCL/fibrin adhesive. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test was performed on the scaffolds to determine cell viability and biocompatibility. The statistical results confirmed that the cell growth is higher on the PCL/fibrin after 4 days.

中文翻译:

静电纺丝法制备聚己内酯/纤维蛋白纳米复合粘合剂的力学和生物学性能

生物聚合物纳米复合材料在组织工程应用中调节细胞功能方面发挥着重要作用。这些纳米复合材料的快速降解和良好的生物活性使其适用于整形外科和医学应用。在这项研究中,聚己内酯 (PCL) 支架和纤维蛋白胶分别用作基质和增强剂。合成了具有不同纵横比的 PCL/纤维蛋白纳米复合材料并用作生物活性粘合剂。PCL/纤维蛋白的拉伸强度使用配备拉伸机的聚合物称重传感器测量。通过场发射扫描电子显微镜研究了 PCL 和 PCL/纤维蛋白纳米复合材料的微观结构分析。微观结构结果表明,在纵横比为 1/10 的 PCL-纤维蛋白纳米复合材料中,具有合适的孔隙率和适当的分布和尺寸。润湿性的结果表明,纤维蛋白作为 PCL 中的增强剂降低了接触角并改善了亲水性。PCL 和 PCL/纤维蛋白支架的粘附特性通过 $$4^\prime $$4' , 6-diamidino-2-phenylindole test 确定。间充质干细胞在 PCL 和 PCL/纤维蛋白支架上培养。结果显示细胞在PCL/纤维蛋白粘合剂上的粘附更多更好。对支架进行了 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑试验,以确定细胞活力和生物相容性。统计结果证实,4 天后 PCL/纤维蛋白上的细胞生长更高。PCL 和 PCL/纤维蛋白支架的粘附特性通过 $$4^\prime $$4' , 6-diamidino-2-phenylindole test 确定。间充质干细胞在 PCL 和 PCL/纤维蛋白支架上培养。结果显示细胞在PCL/纤维蛋白粘合剂上的粘附更多更好。对支架进行了 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑试验,以确定细胞活力和生物相容性。统计结果证实,4 天后 PCL/纤维蛋白上的细胞生长更高。PCL 和 PCL/纤维蛋白支架的粘附特性通过 $$4^\prime $$4' , 6-diamidino-2-phenylindole test 确定。间充质干细胞在 PCL 和 PCL/纤维蛋白支架上培养。结果显示细胞在PCL/纤维蛋白粘合剂上的粘附更多更好。对支架进行了 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑试验,以确定细胞活力和生物相容性。统计结果证实,4 天后 PCL/纤维蛋白上的细胞生长更高。对支架进行了 5-二苯基溴化四唑测试,以确定细胞活力和生物相容性。统计结果证实,4 天后 PCL/纤维蛋白上的细胞生长更高。对支架进行了 5-二苯基溴化四唑试验,以确定细胞活力和生物相容性。统计结果证实,4 天后 PCL/纤维蛋白上的细胞生长更高。
更新日期:2020-06-09
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