SnRK2 protein kinases have been demonstrated to have an important effect on the physiological processes of plant stress tolerance. Here, one of the SnRK2 family members, designated AsSnRK2D, was isolated from oat (Avena sativa Linn. L.). The AsSnRK2D protein includes functional domains specific to the SnRK2 family. The AsSnRK2D protein and its 12 and 21 counterparts were classified into Group II and SnRK2a, respectively. The AsSnRK2D gene was constitutively expressed and positively responded to abscisic acid, low-temperature, salt, and dehydration stress. The subcellular localization analysis revealed that the AsSnRK2.7 protein was localized primarily in the nucleus. Ectopic expression of the AsSnRK2D gene in tobacco resulted in an improvement in the primary root length and plant fresh weight under dehydration and salt stress. The AsSnRK2D-overexpressing tobacco demonstrated improved tolerance to stress resulting from dehydration and salinity, as supported by the morphological changes, survival rates, and physiological indices, including electrolyte leakage, superoxide dismutase level, catalase activity, chlorophyll yield, relative water content, accumulation of reactive oxygen species such as superoxide anion radical and hydrogen peroxide, and the level of free proline. The AsSnRK2D gene was associated with the stress responses of the transgenic tobacco to dehydration and salinity via the regulation of a series of stress-inducible genes, including dehydrins (NtERD10B, NtERD10D, and NtLEA5), cell signaling components (NtPLC3 and NtCaMK1), transcription factors (NtDREB2 and NtDREB4), antioxidative enzymes (NtMnSOD and NtCAT), and proline biosynthesis (NtP5CS). The results show that the AsSnRK2D gene has certain application potential in improving crop tolerances to dehydration and salinity stress.

中文翻译：

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燕麦 SnRK2 基因 AsSnRK2D 的异位表达通过调节胁迫相关基因的表达来增强烟草的脱水和耐盐性

SnRK2 蛋白激酶已被证明对植物胁迫耐受的生理过程具有重要影响。在这里，从燕麦 (Avena sativa Linn. L.) 中分离出一个 SnRK2 家族成员，命名为 AsSnRK2D。AsSnRK2D 蛋白包括特定于 SnRK2 家族的功能域。AsSnRK2D 蛋白及其 12 和 21 对应物分别归入组 II 和 SnRK2a。AsSnRK2D 基因组成型表达，并对脱落酸、低温、盐和脱水胁迫做出积极响应。亚细胞定位分析表明，AsSnRK2.7 蛋白主要定位于细胞核中。AsSnRK2D 基因在烟草中的异位表达导致脱水和盐胁迫下初生根长度和植物鲜重的改善。过表达 AsSnRK2D 的烟草表现出对脱水和盐分胁迫的耐受性提高，这得到形态变化、存活率和生理指标的支持，包括电解质泄漏、超氧化物歧化酶水平、过氧化氢酶活性、叶绿素产量、相对含水量、活性氧，如超氧阴离子自由基和过氧化氢，以及游离脯氨酸的水平。AsSnRK2D 基因通过调控一系列胁迫诱导基因，包括脱水蛋白（NtERD10B、NtERD10D 和 NtLEA5）、细胞信号组分（NtPLC3 和 NtCaMK1）、转录，与转基因烟草对脱水和盐分的胁迫反应相关联。因子（NtDREB2 和 NtDREB4）、抗氧化酶（NtMnSOD 和 NtCAT）和脯氨酸生物合成（NtP5CS）。