Prevention of infection at the surgical area and outbreak of wounds are the most important and at the same time challenging tasks in clinical care. This research investigates the healing effect of laparotomy wounds after the use of bio-fabricated gold nanoparticles (Au NPs) prepared utilizing Thuja occidentalis leaf extract. Ultraviolet–visible (UV–Vis) spectroscopy and X-ray diffraction (X-RD) results confirmed the successful formation of Au NPs. High-resolution Transmission Electron Microscopy (HR-TEM) images confirmed the formation of spherical shaped Au NPs comprising of 30–50 nm particle size. Dermal fibroblasts were subjected to increased concentrations of Au NPs and their effect of cytotoxicity and synthesis of collagen was evaluated. The procedure of celiotomy was conducted on rabbits where the surgical area was exposed topically with either vehicle or once/everyday with Au NPs for about 14 days. Au NPs raised the collagen production from dermal fibroblasts and improved the expression of caspase 3 by exposure to a long period. AuNPs also showed cytotoxic impact with half-maximal inhibitory concentration (IC₅₀) of 0.16 mg/mL. Regular treatment of surgical area with AuNPs led to higher collagen deposition with reduced microbial load and enhanced healing of wounds.

预防外科手术区域的感染和伤口爆发是最重要的，同时也是临床护理中一项艰巨的任务。这项研究调查了使用西方金钟柏叶提取物制备的生物预制金纳米颗粒（Au NPs）后开腹手术伤口的愈合效果。紫外可见（UV-Vis）光谱和X射线衍射（X-RD）结果证实了金纳米颗粒的成功形成。高分辨率透射电子显微镜（HR-TEM）图像证实了球形金纳米颗粒的形成，其粒径为30–50 nm。皮肤成纤维细胞的Au NPs浓度升高，并评估了它们对细胞毒性和胶原蛋白合成的影响。开腹手术是在兔子身上进行的，在兔子的手术区域局部用媒介物或Au NPs每天一次/每天暴露约14天。金纳米颗粒通过长期暴露提高了真皮成纤维细胞的胶原蛋白产量，并改善了胱天蛋白酶3的表达。AuNPs也显示出最大半数抑制浓度的细胞毒性作用（IC ₅₀）0.16 mg / mL。用AuNPs定期治疗手术区域可导致更高的胶原蛋白沉积，降低微生物负荷并增强伤口愈合。