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Mechanistic Modeling of Preparative Column Chromatography for Biotherapeutics.
Annual Review of Chemical and Biomolecular Engineering ( IF 7.6 ) Pub Date : 2020-06-08 , DOI: 10.1146/annurev-chembioeng-102419-125430
Vijesh Kumar 1 , Abraham M Lenhoff 1
Affiliation  

Chromatography has long been, and remains, the workhorse of downstream processing in the production of biopharmaceuticals. As bioprocessing has matured, there has been a growing trend toward seeking a detailed fundamental understanding of the relevant unit operations, which for some operations include the use of mechanistic modeling in a way similar to its use in the conventional chemical process industries. Mechanistic models of chromatography have been developed for almost a century, but although the essential features are generally understood, the specialization of such models to biopharmaceutical processing includes several areas that require further elucidation. This review outlines the overall approaches used in such modeling and emphasizes current needs, specifically in the context of typical uses of such models; these include selection and improvement of isotherm models and methods to estimate isotherm and transport parameters independently. Further insights are likely to be aided by molecular-level modeling, as well as by the copious amounts of empirical data available for existing processes.

中文翻译:


生物治疗学制备柱色谱的机械建模。

色谱法一直以来都是生物制药生产中下游加工的主力军。随着生物处理的成熟,对于相关单元操作寻求详细的基本理解的趋势正在增长,对于某些操作,包括以类似于常规化学过程工业中使用的方式使用机械模型。色谱的机理模型已经开发了近一个世纪,但是,尽管人们普遍理解了基本特征,但是将这些模型用于生物制药加工的专业化包括几个领域,需要进一步阐明。这篇综述概述了这种建模中使用的总体方法,并强调了当前的需求,特别是在这种模型的典型用途的背景下;其中包括等温线模型的选择和改进,以及独立估计等温线和传输参数的方法。分子水平的建模以及现有过程中可用的大量经验数据可能会有助于进一步的洞察。

更新日期:2020-06-08
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