Cervical artery dissection (CAD) is an important causal factor for stroke in young and middle-aged individuals and presents a great burden to the individual stroke victim. However, the pathophysiological mechanisms underlying CAD remain unknown. Here, an iTRAQ (isobaric tagging for relative and absolute quantitation)-based quantitative proteomic approach was performed, to identify differentially expressed proteins in serum samples obtained from spontaneous CAD and non-CAD ischemic stroke subjects. Differential protein expression was analyzed for Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway overrepresentation, and six differential proteins were selected for enzyme-linked immunosorbent assay validation. Through KEGG analysis, the significantly differentiated proteins were primarily involved in immunoregulation, blood coagulation, and lipid metabolism. For the first time, differential expressions of apolipoprotein B, apolipoprotein C-I, lipopolysaccharide-binding protein, vascular cell adhesion molecule 1, fibulin-1, and ficolin-2 were confirmed as being significantly upregulated in CAD as compared to non-CAD ischemic stroke subjects. In conclusion, proteomic analysis reveals that early perturbation of immunoregulation and lipid metabolism may be involved in the pathophysiology of CAD. Specifically, the panel of six proteins identified is promising as serum-based biomarkers for the detection of increased CAD risk in stroke subjects.

中文翻译：

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基于血清的蛋白质组学揭示了中风颈动脉夹层中的脂质代谢和免疫调节失调。

颈动脉夹层（CAD）是导致中青年个体中风的重要原因，并且给个体中风患者带来很大负担。但是，CAD的病理生理机制仍然未知。在这里，进行了基于iTRAQ（等压标记的相对和绝对定量）的定量蛋白质组学方法，以鉴定从自发性CAD和非CAD缺血性中风受试者获得的血清样品中差异表达的蛋白质。分析差异蛋白表达的京都基因和基因组百科全书（KEGG）途径过度表达，并选择了六个差异蛋白用于酶联免疫吸附法验证。通过KEGG分析，明显分化的蛋白质主要参与免疫调节，血液凝固，和脂质代谢。与非CAD缺血性中风受试者相比，首次证实了载脂蛋白B，载脂蛋白CI，脂多糖结合蛋白，血管细胞粘附分子1，fibulin-1和ficolin-2的差异表达在CAD中显着上调。 。总之，蛋白质组学分析表明，早期的免疫调节和脂质代谢紊乱可能与CAD的病理生理有关。具体来说，鉴定出的六种蛋白质的组合有望作为基于血清的生物标志物用于检测中风受试者中增加的CAD风险。与非CAD缺血性中风受试者相比，CAD和Ficolin-2被证实在CAD中明显上调。总之，蛋白质组学分析表明，早期的免疫调节和脂质代谢紊乱可能与CAD的病理生理有关。具体来说，鉴定出的六种蛋白质的组合有望作为基于血清的生物标志物用于检测中风受试者中增加的CAD风险。与非CAD缺血性中风受试者相比，CAD和Ficolin-2被证实在CAD中明显上调。总之，蛋白质组学分析表明，早期的免疫调节和脂质代谢紊乱可能与CAD的病理生理有关。具体来说，鉴定出的六种蛋白质的组合有望作为基于血清的生物标志物用于检测中风受试者中增加的CAD风险。