Abstract

 

Although AD hybrids within the Cryptococcus neoformans species complex represent about 20% of the isolates identified in Europe, phylogenetic and population genetic studies are lacking due to the inability to use the standardized typing method. The aim of the present study was to design new molecular type specific primers in order to apply the standard ISHAM consensus multilocus sequence typing (MLST) scheme to AD hybrids. The new primers are able to specifically amplify VNI and VNIV alleles of the seven MLST loci in both haploid and diploid or aneuploid hybrid strains. This study forms the basis for future molecular epidemiology studies of AD hybrids.

Lay abstract

We designed and tested new specific primers to amplify the two alleles of each of the seven MLST loci in C. neoformans species complex hybrids. The sequences obtained from hybrids can be compared with those present in the Cryptococcus global MLST database for future molecular epidemiology studies.

中文翻译：

---

新的多位点序列分型引物能够对新型隐球菌物种复合体中的 AD 杂种进行基因分型。

摘要

 

尽管新型隐球菌物种复合体中的 AD 杂种约占欧洲鉴定的分离株的 20%，但由于无法使用标准化的分型方法，因此缺乏系统发育和群体遗传学研究。本研究的目的是设计新的分子类型特异性引物，以便将标准 ISHAM 共有多位点序列分型 (MLST) 方案应用于 AD 杂种。新引物能够特异性扩增单倍体和二倍体或非整倍体杂交菌株中七个 MLST 基因座的 VNI 和 VNIV 等位基因。这项研究为未来的 AD 杂种分子流行病学研究奠定了基础。

抽象

我们设计并测试了新的特异性引物，以扩增新生隐球菌物种复杂杂种中七个 MLST 基因座中每一个的两个等位基因。从杂种中获得的序列可以与隐球菌全球 MLST 数据库中的序列进行比较，以用于未来的分子流行病学研究。