ABSTRACT

The main target cells for African swine fever virus (ASFV) replication in pigs are of monocyte macrophage lineage and express markers typical of the intermediate to late stages of differentiation. The lack of a porcine cell line, which accurately represents these target cells, limits research on virus host interactions and the development of live-attenuated vaccine strains. We show here that the continuously growing, growth factor dependent ZMAC-4 porcine macrophage cell line is susceptible to infection with eight different field isolates of ASFV. Replication in ZMAC-4 cells occurred with similar kinetics and to similar high titres as in primary porcine bone marrow cells. In addition we showed that twelve passages of an attenuated strain of ASFV, OURT88/3, in ZMAC-4 cells did not reduce the ability of this virus to induce protection against challenge with virulent virus. Thus, the ZMAC-4 cells provide an alternative to primary cells for ASFV replication.

摘要

猪中非洲猪瘟病毒（ASFV）复制的主要靶细胞是单核细胞巨噬细胞谱系，并表达分化中期到晚期的典型标志物。缺乏能准确代表这些靶细胞的猪细胞系，限制了对病毒宿主相互作用和减毒活疫苗株开发的研究。我们在这里显示，持续增长，依赖生长因子的ZMAC-4猪巨噬细胞细胞系容易感染ASFV的八个不同领域分离株。在ZMAC-4细胞中的复制以与原代猪骨髓细胞相似的动力学和相似的高滴度发生。此外，我们还显示了ASFV减毒株OURT88 / 3的十二个传代，在ZMAC-4细胞中的抗-β-内化酶并没有降低这种病毒诱导抵抗强病毒攻击的能力。因此，ZMAC-4细胞为ASFV复制提供了一种替代原代细胞的方法。