Abstract

The goal of this study was to assess the phenolic composition of the various Ziziphus spina-christi root extracts (aqueous, methanlic, ethanolic and acetonic). The highest amounts of total phenols (60.47 mg GEA/g DW) flavonoids (12.04 mg QE/g DW) and tannin (23.98 mg CE/g DW) were showed in the ethanol solvent. Epicatechin contents were detected, using Liquid Chromatography-mass spectrometry, at level of 6 µg/ml. Furthermore, the concentration of the extract required to scavenge 50% of free radicals (CI 50), investigated by means of DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2-azinobis-(3- ethylbenzothiazoline-6-sulphonate) and Fe²⁺ chelating assays, reached levels of 0.41 ± 0.01; 0.33 ± 0.14 and 0.24 ± 0.03, respectively. The toxic potential against Lasioderma serricorne showed the highest insecticidal potential of Ziziphus spina-christi roots extract. The LC₉₅ values were 3.17 and 4.86 µg/insect respectively for the ethanolic and acetonic extract. The present investigation improves the value of Ziziphus spina-christi as resource of epicathechin, which could be used as bioantioxidant and bioinsecticides.

摘要

这项研究的目的是评估各种Ziziphus spina-christi根提取物（水，甲烷，乙醇和丙酮）中的酚类成分。在乙醇溶剂中，总酚含量最高（60.47 mg GEA / g DW）的黄酮（12.04 mg QE / g DW）和丹宁酸（23.98 mg CE / g DW）最高。使用液相色谱-质谱法检测表儿茶素的含量为6 µg / ml。此外，通过DPPH（2,2-二苯基-1-吡啶基肼基），ABTS（2,2-叠氮基双-（3-乙基苯并噻唑啉- 6-磺酸盐）和Fe ²⁺螯合试验分别达到0.41±0.01、0.33±0.14和0.24±0.03的水平。Lasioderma serricorne表现出最高的Zipziphus spina-christi根提取物的杀虫潜力。乙醇提取物和丙酮提取物的LC ₉₅值分别为3.17和4.86 µg /昆虫。目前的研究提高了刺梨木作为表儿茶素的价值，可以用作生物抗氧化剂和生物杀虫剂。