Abstract

Elastase and tyrosinase are important targets both for cosmetics and for dermatological disorders. In this work, ninety herbal products were tested as inhibitors of these two enzymes. Eleven extracts resulted strongly active. Four out of them (Camellia sinensis, Ginkgo biloba, Rhodiola rosea, Vitis vinifera) inhibited both enzymes, five (Glycyrrhiza glabra, Ribes nigrum, Rheum officinale, Salvia officinalis, Tilia platyphyllos) were active against tyrosinase only, and two (Ceterach officinarum and Cinnamomum zeylanicum) proved selectively active against elastase. The IC₅₀ ranged from 3.1 to 104.9 μg/mL and 19.3 to 164.3 μg/mL, against elastase and tyrosinase, respectively. The most active extracts resulted enriched in flavonoids (from 1.47 to 56.47 mg RE/g of extract) and phenolics (from 37.43 to 123.56 mg GAE/g of extract), indicating also an antioxidant potential. Finally, a positive correlation between enzymatic bioactivities and phenolic content was also established.

摘要

弹性蛋白酶和酪氨酸酶是化妆品和皮肤病的重要靶标。在这项工作中，测试了九十种草药产品作为这两种酶的抑制剂。十一种提取物具有很强的活性。他们四人出来（茶树，银杏，红景天，葡萄）抑制两种酶，五（光果甘草，黑茶藨子，大黄，鼠尾草，椴树platyphyllos）反对酪氨酸酶的活性只，和两个（Ceterach良姜和肉桂）被证明对弹性蛋白酶具有选择性的活性。针对弹性蛋白酶和酪氨酸酶的IC _50分别为3.1至104.9μg/ mL和19.3至164.3μg/ mL。活性最强的提取物富含类黄酮（从1.47到56.47 mg RE / g提取物）和酚类（从37.43到123.56 mg GAE / g提取物）富集，也表明具有抗氧化潜力。最后，还建立了酶促生物活性与酚含量之间的正相关关系。