Cover photograph : Reverse genetics system for human rotaviruses (HuRVAs). BHK/T7‐9 cells constitutively expressing T7 RNA polymerase are transfected with a set of 11 rescue T7 plasmids encoding a HuRVA genome with a 3‐fold increased quantity ratio of rescue T7 plasmids encoding NSP2 and NSP5 segments. Recombinant HuRVAs can be recovered after propagation using a high trypsin concentration and the roller‐tube culture technique. PT7 and Rib denote the T7 RNA polymerase promoter and hepatitis delta virus ribozyme sequence, respectively. Microbiol Immunol: 64:401–406 . Article link here

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发行信息–封面

封面照片：人类轮状病毒（HuRVAs）的反向遗传学系统。用一组11个编码HuRVA基因组的救援T7质粒转染组成型表达T7 RNA聚合酶的BHK / T7-9细胞，其编码NSP2和NSP5片段的救援T7质粒的数量比增加3倍。重组的HuRVAs可以在繁殖后使用高浓度的胰蛋白酶和滚轮培养技术进行回收。PT7和Rib分别表示T7 RNA聚合酶启动子和肝炎三角洲病毒核酶序列。微生物免疫：64：401–406。文章链接在这里