The region‐specific expression of seminal proteins in testis and excurrent duct system determines the quality and function of the spermatozoa. In the present study, localization and expression of some of the seminal proteins such as insulin‐like growth factor receptor 1β (IGF‐1Rβ), phosphatidylethanolamine‐binding protein 4 (PEBP4), α‐tubulin and tissue factor pathway inhibitor 2 (TFPI2) were carried out in testis, excurrent duct system and spermatozoa of buffalo. IGF‐1Rβ was localized in the cells of the seminiferous tubules of the testis, except in primary spermatocytes. The PEBP4 was localized only in the elongated spermatid, whereas α‐tubulin and TFPI2 proteins were localized in all cells of the seminiferous tubule including spermatocyte. In the buffalo spermatozoa, IGF‐1Rβ, PEBP4, α‐tubulin and TFPI2 were localized in the acrosome region, the post‐acrosomal region till the tail end, post‐acrosome to the entire tail region and the equatorial region, respectively. The study indicates that IGF‐1R, α‐tubulin and PEBP4 proteins regulate spermatogenesis, whereas TFPI2 may be involved during the zona binding process of the buffalo spermatozoa.

中文翻译：

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免疫组织学标测和睾丸和精液中精液蛋白与水牛精子功能的功能联系。

精巢蛋白在睾丸和导管系统中的区域特异性表达决定了精子的质量和功能。在本研究中，一些精蛋白的定位和表达，如胰岛素样生长因子受体1β（IGF-1Rβ），磷脂酰乙醇胺结合蛋白4（PEBP4），α-微管蛋白和组织因子途径抑制剂2（TFPI2）在睾丸，外管系统和水牛精子中进行。IGF-1Rβ定位在睾丸生精小管的细胞中，除了原代精母细胞。PEBP4仅位于细长的精子细胞中，而α-微管蛋白和TFPI2蛋白则位于生精小管的所有细胞（包括精细胞）中。在水牛精子中，IGF-1Rβ，PEBP4，α-微管蛋白和TFPI2位于顶体区域，顶体后区域直到尾端，顶体后分别到整个尾巴区域和赤道区域。研究表明，IGF-1R，α-微管蛋白和PEBP4蛋白调节精子发生，而TFPI2可能参与水牛精子的透明带结合过程。