Food safety assessment of crops engineered with RNA interference and other methods to modulate expression of endogenous and plant pest genes.,Pest Management Science

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Food safety assessment of crops engineered with RNA interference and other methods to modulate expression of endogenous and plant pest genes.
Pest Management Science ( IF 3.8 ) Pub Date : 2020-06-09 , DOI: 10.1002/ps.5957
Gijs A Kleter ₁

Affiliation

Genetically modified crops have been grown commercially for more than two decades. Some of these crops have been modified with genetic constructs that induce gene silencing through RNA interference (RNAi). The targets for this silencing action are genes, either specific endogenous ones of the host plant or those of particular pests or pathogens infesting these plants. Recently emerging new genetic tools enable precise DNA edits with the same silencing effect and have also increased our knowledge and insights into the mechanisms of RNAi. For the assessment of the safety of foodstuffs from crops modified with RNAi, internationally harmonized principles for risk assessment of foods derived from genetically modified crops can be followed. Special considerations may apply to the newly expressed silencing RNA molecules, such as their possible uptake by consumers and interference with expression of host genes, which, however, would need to overcome many barriers. Bioinformatics tools aid the prediction of possible interference by a given RNA molecule with the expression of genes with homologous sequences in the host crop and in other organisms, or possible off‐target edits in gene‐edited crops. © 2020 The Author. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

中文翻译：

利用RNA干扰和其他方法调节内源和植物有害生物基因表达的农作物的食品安全性评估。

转基因作物已经商业化种植了二十多年。这些作物中的一些已经用遗传构建物修饰，这些构建物通过RNA干扰（RNAi）诱导基因沉默。这种沉默作用的靶标是基因，是宿主植物的特定内源基因，或是侵害这些植物的特定害虫或病原体的基因。最近出现的新遗传工具能够以相同的沉默效果进行精确的DNA编辑，并且还增加了我们对RNAi机理的知识和见识。为了评估使用RNAi修饰的农作物食品的安全性，可以遵循国际统一的原则，对源自转基因农作物的食品进行风险评估。特殊注意事项可能适用于新表达的沉默RNA分子，例如可能被消费者吸收，以及干扰宿主基因的表达，但这将需要克服许多障碍。生物信息学工具可帮助预测给定的RNA分子可能会对宿主作物和其他生物中具有同源序列的基因表达产生干扰，或在基因编辑作物中可能产生脱靶编辑。©2020作者。John Wiley＆Sons Ltd代表化学工业协会出版的《害虫管理科学》。

更新日期：2020-06-09

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