The CWPS Rubik's cube: Linking diversity of cell wall polysaccharide structures with the encoded biosynthetic machinery of selected Lactococcus lactis strains.,Molecular Microbiology

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The CWPS Rubik's cube: Linking diversity of cell wall polysaccharide structures with the encoded biosynthetic machinery of selected Lactococcus lactis strains.
Molecular Microbiology ( IF 2.6 ) Pub Date : 2020-06-09 , DOI: 10.1111/mmi.14561
Jennifer Mahony _{1,

2} , Cyril Frantzen ₃ , Evgeny Vinogradov ₄ , Irina Sadovskaya ₅ , Ilias Theodorou ₁ , Philip Kelleher _{1,

2} , Marie-Pierre Chapot-Chartier ₆ , Christian Cambillau _{1,

7} , Helge Holo ₃ , Douwe van Sinderen _{1,

2}

Affiliation

The biosynthetic machinery for cell wall polysaccharide (CWPS) production in lactococci is encoded by a large gene cluster, designated cwps. This locus displays considerable variation among lactococcal genomes, previously prompting a classification into three distinct genotypes (A–C). In the present study, the cwps loci of 107 lactococcal strains were compared, revealing the presence of a fourth cwps genotype (type D). Lactococcal CWPSs are comprised of two saccharidic structures: a peptidoglycan‐embedded rhamnan backbone polymer to which a surface‐exposed, poly/oligosaccharidic side‐chain is covalently linked. Chemical structures of the side‐chain of seven lactococcal strains were elucidated, highlighting their diverse and strain‐specific nature. Furthermore, a link between cwps genotype and chemical structure was derived based on the number of glycosyltransferase‐encoding genes in the cwps cluster and the presence of conserved genes encoding the presumed priming glycosyltransferase. This facilitates predictions of several structural features of lactococcal CWPSs including (a) whether the CWPS possesses short oligo/polysaccharide side‐chains, (b) the number of component monosaccharides in a given CWPS structure, (c) the order of monosaccharide incorporation into the repeating units of the side‐chain (for C‐type strains), (d) the presence of Galf and phosphodiester bonds in the side‐chain, and (e) the presence of glycerol phosphate substituents in the side‐chain.

中文翻译：

CWPS Rubik魔方：将细胞壁多糖结构的多样性与选定的乳酸乳球菌菌株的编码生物合成机制联系起来。

所述生物合成机构用于乳球菌细胞壁多糖（CWPS）的生产是由一个大基因簇，指定编码CWPS。该基因座在乳球菌基因组之间显示出相当大的差异，从而促使人们将其分类为三种不同的基因型（A–C）。在本研究中，比较了107个乳球菌菌株的cwps基因座，发现存在第四个cwps基因型（D型）。乳球菌CWPS由两个糖结构组成：肽聚糖包埋的鼠李聚糖主链聚合物，表面暴露的聚/寡糖侧链与之共价连接。阐明了七个乳球菌菌株侧链的化学结构，突出了它们的多样性和菌株特异性。此外，基于cwps中糖基转移酶编码基因的数量，得出了cwps基因型与化学结构之间的联系簇和编码推测的启动糖基转移酶的保守基因的存在。这有助于预测乳球菌CWPS的几个结构特征，包括（a）CWPS是否具有短寡糖/多糖侧链，（b）给定CWPS结构中单糖组分的数量，（c）单糖掺入CWPS的顺序。侧链的重复单元（对于C型菌株），（d）侧链中存在Gal f和磷酸二酯键，以及（e）侧链中存在磷酸甘油酯取代基。

更新日期：2020-06-09

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