Hypoderma antigens are involved in host inflammation and immune response, conditioning larvae survival. In this study, peripheral blood mononuclear cell (PBMC) cultures from Hypoderma sensitized and unsensitized cattle were performed to determine the effect of H. lineatum antigens and incubation time (18, 24, 48 h) on IFN-γ, TNF-α, IL-10 and IL-4 mRNA gene expression determined by RT-qPCR. TNF-α and IL-4 gene expression were higher in Hypoderma previously sensitized PBMCs, suggesting that a mixed Th1/Th2 response may play a significant role in host defence reactions against Hypoderma exhibited by previously infested cattle. Incubation time had a significant effect on IL-10 and TNF-α gene expression, which decreased over time. Regarding to H. lineatum antigens, the crude larval extract and the purified fraction hypodermin B (HB) produced a significant reduction of the mRNA expression levels of the proinflammatory cytokine, IFN-γ; moreover, the HB had a stimulating effect on the mRNA gene expression of the anti-inflammatory cytokine IL-10, demonstrating that the parasite would modulate the host defence mechanisms by avoiding harmful immune responses that would limit its survival into the host tissues.

皮下抗原参与宿主炎症和免疫反应，调节幼虫存活。在这项研究中，从Hypoderma致敏和未致敏的牛的外周血单个核细胞（PBMC）培养进行，以确定H.lineatum抗原和孵育时间（18、24、48 h）对IFN-γ，TNF-α，IL的影响通过RT-qPCR测定-10和IL-4 mRNA的表达。以前皮下过敏的PBMC中TNF-α和IL-4基因表达较高，表明混合的Th1 / Th2反应可能在针对皮下的宿主防御反应中起重要作用由先前被感染的牛展示。孵育时间对IL-10和TNF-α基因表达有显着影响，并随时间而降低。关于H. lineatum抗原，粗幼虫提取物和纯化级分次皮蛋白B（HB）使促炎细胞因子IFN-γ的mRNA表达水平显着降低。而且，HB对抗炎细胞因子IL-10的mRNA基因表达具有刺激作用，表明该寄生虫将通过避免有害的免疫反应而调节宿主的防御机制，有害的免疫反应会限制其在宿主组织中的存活。