Abstract In the present study, environmentally benign silver nanoparticles were synthesized using commercially purchased shrimp-shell chitosan as a capping agent. The synthesized chitosan-silver nanoparticles (Ch-AgNPs) were physico-chemically characterized by UV–Vis spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), high-resolution transmission electron microscopy (HR-TEM) along with energy dispersive X-ray analysis (EDX), DLS and Zeta potential analysis. Ch-Ag NPs were crystalline, uniformly dispersed, and spherically shaped, with particle size between 8 and 48 nm. The average size of Ch-AgNPs was 21 nm. In-vitro anti-biofilm activity of Ch-AgNPs was tested against wound infection-causing pathogenic bacteria such as Staphylococcus aureus (Gram-positive) and Pseudomonas aeruginosa (Gram-negative). Ch-AgNPs displayed anti-biofilm activity in a dose-dependent manner. Light and confocal-laser scanning microscopy confirmed the significant inhibition of biofilm growth of S. aureus (85%) and P. aeruginosa (95%) at 100 μg mL−1 of Ch-AgNPs. Moreover, Ch-AgNPs promoted wound healing by increasing the migration of RAW 264.7 murine macrophages cells at 75 and 100 μg mL−1after 24 h. In addition, in vitro cytotoxicity of Ch-AgNPs against MCF 7 (human breast cancer) cells, depicted the greater inhibition of proliferation of cells (64%) at 100 μg mL−1.

中文翻译：

---

壳聚糖包覆的银纳米粒子促进鼠巨噬细胞的抗菌、抗生物膜、伤口愈合和人乳腺癌 MCF7 细胞的抗增殖

摘要 在本研究中，使用商业购买的虾壳壳聚糖作为封端剂合成了对环境无害的银纳米粒子。合成的壳聚糖-银纳米粒子 (Ch-AgNPs) 通过紫外-可见光谱、X 射线衍射 (XRD)、傅里叶变换红外光谱 (FTIR)、高分辨率透射电子显微镜 (HR-TEM) 进行物理化学表征。具有能量色散 X 射线分析 (EDX)、DLS 和 Zeta 电位分析。Ch-Ag NPs 是结晶的、均匀分散的、球形的，粒径在 8 到 48 nm 之间。Ch-AgNPs 的平均尺寸为 21 nm。对 Ch-AgNPs 的体外抗生物膜活性进行了针对引起伤口感染的病原菌，如金黄色葡萄球菌（革兰氏阳性）和铜绿假单胞菌（革兰氏阴性）的测试。Ch-AgNPs 以剂量依赖性方式显示出抗生物膜活性。光学和共聚焦激光扫描显微镜证实了在 100 μg mL-1 的 Ch-AgNPs 下，金黄色葡萄球菌 (85%) 和铜绿假单胞菌 (95%) 的生物膜生长受到显着抑制。此外，Ch-AgNPs 通过在 24 小时后以 75 和 100 μg mL-1 增加 RAW 264.7 鼠巨噬细胞的迁移来促进伤口愈合。此外，Ch-AgNPs 对 MCF 7（人乳腺癌）细胞的体外细胞毒性表明，100 μg mL-1 时对细胞增殖的抑制作用更大（64%）。24 小时后 75 和 100 μg mL-1 的 7 个鼠巨噬细胞。此外，Ch-AgNPs 对 MCF 7（人乳腺癌）细胞的体外细胞毒性表明，100 μg mL-1 时对细胞增殖的抑制作用更大（64%）。24 小时后 75 和 100 μg mL-1 的 7 个鼠巨噬细胞。此外，Ch-AgNPs 对 MCF 7（人乳腺癌）细胞的体外细胞毒性表明，100 μg mL-1 时对细胞增殖的抑制作用更大（64%）。