An experiment was conducted to evaluate responses of broiler chickens fed a corn-soy (soy protein isolate) choline-deficient diet that was gradually supplemented with choline chloride to a content expected to exceed usual choline concentrations. A total of 525 one-day-old slow feathering male chicks were randomly distributed to 5 treatments and 15 replicates of 7 broiler chickens each. Broilers were fed diets having dietary choline supplementations at 0, 700, 1,400, 2,100 and 2,800 ppm from choline chloride, totalizing formulated concentrations at 727, 1,427, 2,127, 2,827 and 3,527 ppm, respectively. The non-supplemented diet had 736 ppm analyzed choline. Growth performance was weekly evaluated until d 21 when leg deviations were evaluated and liver and tibia were collected. Broiler chicken responses were fitted to quadratic polynomial (QP) and quadratic broken-line (QBL) models. Broilers fed the diet without choline supplementation had the lowest BWG and FI, greaterr FCR as well as greater varus and rotated tibia frequency (P < 0.05) compared to broiler chickens fed diets at any supplemental concentration of choline. Estimations of maximum responses for choline requirements from d 1 to 21 using the QP and QBL regressions were of 2,936 and 2,077 ppm for BWG and 2,827 and 1,627 ppm for FCR, respectively. Broiler chickens fed the diet without choline chloride supplementation had the greatest varus (5.7%) and rotated tibia scores (19.3%) (P < 0.01). The average of total choline requirement estimates obtained were 2,516 ppm for BWG and 2,533 ppm for FCR, whereas 2,202 ppm was needed to minimize the locomotor disorders.

进行了一项实验，以评估饲喂玉米大豆（大豆蛋白分离物）胆碱缺乏日粮的肉鸡的反应，该日粮中逐渐添加氯化胆碱使其含量超过通常的胆碱浓度。将总共​​525只1天大的慢羽雄性小鸡随机分配给5种处理方法，每7只肉鸡进行15次重复。肉鸡饲喂饮食中胆碱氯化物的添加量分别为氯化胆碱的0、700、1,400、2,100和2,800 ppm，配制的总浓度分别为727、1,427、2,127、2,827和3,527 ppm。非补充饮食的胆碱含量为736 ppm。每周评估生长性能，直到第21天评估腿部偏离并收集肝脏和胫骨。肉鸡的反应适合二次多项式（QP）和二次虚线（QBL）模型。与饲喂任何补充浓度胆碱的肉鸡相比，饲喂不添加胆碱的肉鸡的BWG和FI最低，FCR更大，内翻和胫骨旋转频率更高（P <0.05）。使用QP和QBL回归估计的d 1至21胆碱需求的最大响应分别为BWG为2,936和2,077 ppm，而FCR为2,827和1,627 ppm。饮食中未添加氯化胆碱的肉鸡内翻最大（5.7％），胫骨旋转评分最大（19.3％）（P <0.01）。获得的总胆碱需求估计值的平均值对于BWG为2,516 ppm，对于FCR为2,533 ppm，而2，