Abstract Determination of feline meat in food products is an important issue for social, health, economic and religious concern. Hence this paper documented the application of species specific polymerase chain reaction-restriction fragment length polymorphism (SP-PCR-RFLP) assay targeting a short-fragments (69 bp) of mitochondrial cytochrome b (cytb) gene to screen feline meat in commercial meat products using lab-on-a-chip. The SP-PCR assay proved its specificity theoretically and experimentally while testing with different common animal, aquatic and plant species of DNA. The feline specific (69 bp, 43- and 26-bp) characteristic molecular DNA pattern was observed by SP-PCR and RFLP analysis. For assay performance, it was tested in three different types of commercial dummy meat products such as frankfurters, nuggets and meatballs and digested with AluI-restriction enzyme. The highest sensitivity of the assay using lab-on-a-chip was as low as 0.1 pg or 0.01% (w/w) in commercial dummy meat products. We have also applied this assay to screen three important commercial meat products of six different brand from six supermarket chains located at three different states of Malaysia. Thus total 378 samples were tested to validate the specificity, sensitivity, stability of the assay and utilization of it for commercial meat product screening.

中文翻译：

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使用 SP-PCR-RLFP 和芯片实验室从超市连锁店筛选商业肉类产品中的猫科动物衍生物

摘要 食品中猫肉的测定是社会、健康、经济和宗教关注的重要问题。因此，本文记录了针对线粒体细胞色素 b (cytb) 基因的短片段 (69 bp) 的物种特异性聚合酶链反应限制性片段长度多态性 (SP-PCR-RFLP) 检测在商业肉制品中筛选猫肉的应用使用芯片实验室。SP-PCR 检测在用不同的常见动物、水生和植物物种 DNA 进行测试时，从理论上和实验上证明了其特异性。通过 SP-PCR 和 RFLP 分析观察到猫特异性（69 bp、43 和 26 bp）特征分子 DNA 模式。对于测定性能，它在三种不同类型的商业假肉产品中进行了测试，例如法兰克福香肠、块和肉丸，并用 AluI 限制酶消化。使用芯片实验室检测的最高灵敏度在商业人造肉制品中低至 0.1 pg 或 0.01% (w/w)。我们还应用此检测方法筛选了来自马来西亚三个不同州的六家连锁超市的六种不同品牌的三种重要商业肉类产品。因此，总共测试了 378 个样品，以验证检测的特异性、灵敏度、稳定性及其在商业肉类产品筛选中的应用。我们还应用此检测方法筛选了来自马来西亚三个不同州的六家连锁超市的六种不同品牌的三种重要商业肉类产品。因此，总共测试了 378 个样品，以验证检测的特异性、灵敏度、稳定性及其在商业肉类产品筛选中的应用。我们还应用此检测方法筛选了来自马来西亚三个不同州的六家连锁超市的六种不同品牌的三种重要商业肉类产品。因此，总共测试了 378 个样品，以验证检测的特异性、灵敏度、稳定性及其在商业肉类产品筛选中的应用。