A technique of preparative adsorption chromatography, according to which the separation of substances is determined by the effect of kinetic selectivity of sorption (KSS), is discussed. Unlike traditional chromatography methods, in which separation is determined by the difference in the binding strength of the separated components (distribution coefficients), in kinetically selective chromatography, the separation parameters can be controlled by changing the ratios between the distribution coefficients and the sorption rate of components (effective diffusion coefficients). The necessary and sufficient prerequisites for the inversion of the selectivity of sorption of the components of sorption systems are identified, when the order of components coming off the column is reversed and a component with a higher equilibrium distribution coefficient comes out of the column first. The KSS effect consists in the fact that a component with a higher distribution coefficient is more selectively adsorbed before the selectivity inversion point and a component with a lower distribution coefficient is preferably absorbed after the inversion point. The prospects of practical use of kinetically selective chromatography are considered using systems important for biotechnology and pharmaceutics. The use of the KSS effect allowed us to optimize the separation of a number of biologically active substances both in the case of the inversion of sorption selectivity of the separated components and in those cases when the conditions of the inversion of sorption selectivity were not met.

中文翻译：

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聚合物吸附剂上生物活性物质的动力学选择性制备色谱

讨论了一种制备吸附色谱技术，根据该技术，物质的分离取决于吸附动力学选择性（KSS）的影响。不同于传统的色谱方法，分离是由被分离组分的结合强度（分布系数）的差异决定的，在动力学选择性色谱中，分离参数可以通过改变分布系数和吸附速率之间的比率来控制。成分（有效扩散系数）。确定了吸附系统组分的吸附选择性反转的必要和充分的先决条件，当从塔中出来的组分的顺序颠倒并且具有较高平衡分配系数的组分首先从塔中出来时。KSS效应在于具有较高分配系数的组分在选择性反转点之前被更有选择性地吸附，而具有较低分配系数的组分优选在反转点之后被吸收。使用对生物技术和制药学很重要的系统，考虑了动力学选择性色谱的实际应用前景。