Begonia semperflorens-cultorum, known as wax begonia, is one of the most popular Begonia species in which variable commercial cultivars have been produced. The genetic transformation technique is required for further modification of this species because introduction of some desired traits such as novel flower colors cannot be achieved by conventional breeding. Here we report the procedures of an efficient plant regeneration from leaf segment and production of transgenic plants using Begonia semperflorens-cultorum. Efficient induction of adventitious shoots was achieved when the explants were cultured on MS medium supplemented with 1.5 mg/l thidiazuron (TDZ), 0.5 mg/l α-naphthaleneacetic acid (NAA), 30 g/l sucrose and 2.5 mg/l gellan gum. Agrobacterium tumefaciens strain EHA101 containing the plasmid pIG121-Hm was used for gene transfer. The transient GUS expression was significantly increased when 10 mM MES was added to the co-cultivation medium. By culturing the infected explants under the selection pressure with 10 mg/l hygromycin (Hm), the Hm-resistant independent shoots were obtained at a frequency of 0.78 per explant. The regenerated Hm-resistant plants showed the integration of hygromycin phosphotransferase (HPT) gene into the genome and stable GUS expression, as the proof of genetic transformation. Consequently, the conditions established in this study enabled the transformation of Begonia semperflorens-cultorum.

秋海棠（Segerflorens-cultorum），被称为蜡秋海棠，是最受欢迎的秋海棠物种之一，其中已经产生了多种商业化品种。需要遗传转化技术来进一步修饰该物种，因为常规育种无法实现某些所需性状的引入，例如新颖的花色。在这里，我们报告从叶节有效植物再生的程序和使用秋海棠-秋海棠转基因植物的生产。当外植体在补充有1.5 mg / l噻唑隆（TDZ），0.5 mg / lα-萘乙酸（NAA），30 g / l蔗糖和2.5 mg / l结冷胶的MS培养基上培养时，可以有效诱导不定芽。 。含有质粒pIG121-Hm的根癌农杆菌菌株EHA101用于基因转移。当将10 mM MES添加到共培养培养基中时，瞬时GUS表达显着增加。通过在选择压力下用10 mg / l潮霉素（Hm）培养受感染的外植体，以每株外植体0.78的频率获得抗Hm的独立芽。再生的抗Hm植株显示潮霉素磷酸转移酶（HPT）基因已整合到基因组中并稳定了GUS表达，这是遗传转化的证明。因此，在这项研究中建立的条件能够实现四季海棠的转化。