The potential of analytical ultracentrifugation (AUC) in the analysis of the drug distribution of liposomal doxorubicin formulation (with nominal diameter of 85 nm) in the presence of human serum proteins is demonstrated using the absorbance detection function of the instrument. Based on the AUC measurement (and model fitting for molecular mass calculation), we show that in a single experiment, it is possible to measure the relative amounts of the free drug, of the liposome-encapsulated drug, and of the serum protein-bound drug. In addition, the same data provides both the accurate particle size distribution of the liposomal formulation in human serum and information on the protein that binds doxorubicin in the drug-protein fraction (in this case, human serum albumin). Thus, a single experiment (that requires only minimal sample preparation) provides several critical physical-chemical attributes of liposomal drug formulations. This innovative approach will greatly help in the development of improved methods for the challenging problem of characterizing nanomedicine in relevant biological matrices.

使用仪器的吸光度检测功能证明了在人血清蛋白存在下分析超速离心（AUC）在分析脂质体阿霉素制剂（标称直径为85 nm）的药物分布中的潜力。基于AUC测量（和用于分子质量计算的模型拟合），我们表明在单个实验中，可以测量游离药物，脂质体包裹的药物和结合血清蛋白的相对量药品。另外，相同的数据既提供了脂质体制剂在人血清中的准确粒径分布，又提供了与药物-蛋白级分中的阿霉素结合的蛋白（在这种情况下，是人血清白蛋白）的信息。从而，单个实验（仅需要最少的样品制备）即可提供脂质体药物制剂的几个关键的物理化学属性。这种创新方法将极大地帮助开发改进的方法，以解决相关生物基质中表征纳米药物的难题。