Franklinia is a monotypic genus of the family Theaceae that is now extinct in the wild. F. alatamaha Bartram ex Marshall has been maintained in cultivation for nearly 250-y because of the ornamental appeal of its flowers and foliage. The goal of this research was to develop an in vitro propagation system to increase the availability of this rare tree for commercial horticulture and restoration. Immature zygotic embryos were cultured on semi-solid Woody Plant Medium containing 34 different plant growth regulator treatments. Shoot organogenesis was induced from dark-grown explants with indole-3-butyric acid in combination with 6-benzylaminopurine. Shoot organogenesis was also obtained with thidiazuron, N-(2-Chloro-4-pyridyl)-N′-phenylurea, and indole-3-butyric acid in combination with meta-Topolin, although shoot development was abnormal. Adventitious buds were produced on the epidermal surfaces of immature zygotic embryos while exposed to the above plant growth regulator treatments. Once the adventitious buds elongated into shoots, these adventitious shoots were further multiplied by axillary shoot production. Elongated shoots from axillary shoot production were rooted on Woody Plant Medium supplemented with 0.25 g L⁻¹ activated charcoal, but no plant growth regulators. The resulting plantlets were transferred to soil and acclimatized to the greenhouse and outdoor environments. Tissue culture-derived trees flowered within one and a half years in the greenhouse under an extended photoperiod.

富兰克林（Franklinia）是Theaceae家族的单型属，现已在野外灭绝。F. alatamaha Bartram ex Marshall因其花朵和叶子的装饰魅力而在栽培中保持了近250年的历史。这项研究的目的是开发一种体外繁殖系统，以增加这种稀有树用于商业园艺和恢复的可用性。未成熟的合子胚在含有34种不同植物生长调节剂处理剂的半固态木本植物培养基上培养。用吲哚-3-丁酸与6-苄基氨基嘌呤结合，从深色外植体中诱导出芽器官发生。噻噻隆，N-（2-氯-4-吡啶基）-N也获得了芽器官发生。尽管芽发育异常，但'-苯基脲和吲哚-3-丁酸与间-Topolin结合。当暴露于上述植物生长调节剂处理时，不定芽在未成熟合子胚的表皮表面上产生。一旦不定芽伸长成芽，这些不定芽再乘以腋芽的繁殖。来自腋生芽的细长芽生根于补充有0.25 g L ^-1活性炭但没有植物生长调节剂的木质植物培养基上。将所得的小植株转移到土壤中并使其适应温室和室外环境。来自组织培养的树木在延长的光周期下于温室中开花一年半。