The establishment of green root cultures of Stevia rebaudiana Bertoni, and the effect of elicitors such as hydrogen peroxide (H₂O₂) and methyl jasmonate (MeJA), is shown in the present study. Stevioside, rebaudioside A, and the isomers steviol/isosteviol were identified through DFI-ESI-IT-MSⁿ and UPLC-TOFMS spectrometric systems, in combination with solid-phase extraction. The accumulation of steviol glycosides increased by 2.4 times (compared to the control value of 22.35 μgSG per gDW), with the addition of 250 μM H₂O₂. The non-enzymatic antioxidant response, which resulted from production of phenolic and flavonoid compounds, was modified based on the elicitor and the dose used. The maximum accumulation of flavonoids was induced on the third day with the addition of H₂O₂ (250 or 500 μM), and with MeJA (250 or 500 μM); the increase was observed on the fifth day. The enzymatic antioxidant response of the catalase and peroxidase from the roots under elicitation confirmed the stress conditions.

本研究显示了甜叶菊甜绿根培养物的建立，以及过氧化氢（H ₂ O ₂）和茉莉酸甲酯（MeJA）等引发剂的作用。通过DFI-ESI-IT-MS ⁿ和UPLC-TOFMS光谱系统结合固相萃取，鉴定了甜菊糖苷，莱鲍迪苷A和甜菊醇/异甜菊醇的异构体。添加250μMH ₂ O _2时，甜菊醇糖苷的积累增加了2.4倍（相比之下，每gDW的对照值为22.35μgSG）。。根据引发剂和所用剂量，对酚类和类黄酮化合物产生的非酶促抗氧化反应进行了修饰。加入H ₂ O ₂（250或500μM）和MeJA（250或500μM）的第三天，诱导了类黄酮的最大积累；在第五天观察到增加。来自根的过氧化氢酶和过氧化物酶的酶促抗氧化反应在诱导下证实了胁迫条件。