Plants were regenerated from in vitro leaf and petiole tissues of Rubus spp. L. (red raspberry) ‘Joan J’ and ‘Polana’ in woody plant medium (WPM), with 200 mg L⁻¹ polyvinylpyrrolidone (PVP), and various combinations of 6-benzylaminopurine (BA) and/or thidiazuron (TDZ). The effects of explant type and explant age on plant regeneration were also investigated. Results showed that the regeneration rates were 70% for ‘Joan J’ and 82.2% for ‘Polana,’ when 7-d-old leaf tissues were inoculated on WPM with 2.5 μM BA + 1.0 μM TDZ and WPM with 2.5 μM BA + 0.1 μM TDZ, respectively. For ‘Joan J,’ in vitro rooting was readily achieved (100%) in one-half Murashige and Skoog (MS) medium supplemented with 10 μM 1-naphthaleneacetic acid (NAA). For ‘Polana,’ a rooting rate of 75% was achieved when cuttings were pulsed in one-half MS with 20 μM NAA for 7 d and then transferred to auxin-free one-half MS medium. An efficient ex vitro rooting frequency (> 80%) was obtained for both cultivars when cuttings were quickly dipped in 500 μM Indole-3-burytic acid (IBA) or NAA, and inserted to Sunshine Mix no. 1 (Fisons Western Corp., Vancouver, Canada) for a 4-wk rooting and acclimatization period. Regenerated plants were eventually grown in the greenhouse. This research will assist germplasm improvement using biotechnology techniques for raspberries and other Rubus species.

从Rubus spp的体外叶和叶柄组织再生植物。L.（红树莓）“ Joan J”和“ Polana”在木本植物培养基（WPM）中，含200 mg L ^-1聚乙烯吡咯烷酮（PVP）和6-苄基氨基嘌呤（BA）和/或噻唑酮（TDZ）的各种组合。还研究了外植体类型和外植体年龄对植物再生的影响。结果表明，当在2.5毫米BA + 1.0微米TDZ的WPM和2.5毫米BA + 0.1的WPM上接种7天大的叶片组织时，“琼J”的再生率为70％，“波拉纳”的再生率为82.2％。分别为μMTDZ。对于“琼J”，在体外在补充了10μM1-萘乙酸（NAA）的一半Murashige和Skoog（MS）培养基中很容易生根（100％）。对于'Polana'，当将插条在含20μMNAA的一半MS中脉冲处理7 d，然后转移到无生长素的一半MS培养基中时，生根率达到75％。当将插条快速浸入500μM吲哚-3-硬脂酸（IBA）或NAA中，并插入到Sunshine Mix no。2中时，两个品种均获得了有效的离体生根频率（> 80％）。1（Fisons Western Corp.，加拿大温哥华），为期4周生根和适应。再生植物最终在温室中生长。这项研究将使用生物技术改善覆盆子和其他悬钩子的种类，帮助改良种质。