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Effect of exogenous application of IAA and GA 3 on growth, protein content, and antioxidant enzymes of Solanum tuberosum L. grown in vitro under salt stress
In Vitro Cellular & Developmental Biology - Plant ( IF 2.2 ) Pub Date : 2020-01-13 , DOI: 10.1007/s11627-019-10047-x
Arifa Khalid , Faheem Aftab

Indole-3-acetic acid (IAA) and gibberellic acid (GA3) are essential for the growth and development of plants. In the present study, the ameliorative potential of these phytohormones on growth, protein content, and antioxidant enzymes was investigated in in vitro-grown Solanum tuberosum L. cultivars ‘Cardinal’ and ‘Desiree’ under salt stress. A 4 × 3 factorial combination of 0, 40, 60, or 80 mM NaCl with 0, 7, or 14 μM IAA, or 0, 14, or 21 μM GA3, were added to Murashige and Skoog (MS) basal medium, followed by inoculation of nodal explants or callus cultures. The data for root and shoot number and length, number of nodes and leaves, fresh weight of plants, increase or decrease in fresh weight of callus cultures, total soluble protein, and superoxide dismutase (SOD) and peroxidase (POD) activities were recorded after 30 d. The growth of both callus cultures and nodal explants subjected to NaCl stress was substantially reduced compared with the control. Both IAA and GA3 successfully alleviated the harmful effects of salt stress on all of the growth parameters studied. Salt stress resulted in decreased protein content, which increased when the media also contained phytohormones. The activities of SOD and POD were increased with either IAA or GA3 under NaCl stress. Therefore, the exogenous application of both IAA and GA3 not only played a positive role in terms of in vitro potato growth but also significantly affected the biochemical parameters tested.



中文翻译:

外源施用IAA和GA 3对盐胁迫下体外培养的马铃薯的生长,蛋白质含量和抗氧化酶的影响

吲哚-3-乙酸(IAA)和赤霉素(GA 3)对植物的生长和发育至关重要。在本研究中,研究了盐胁迫下体外培养的马铃薯(Solanum tuberosum L.)品种'Cardinal'和'Desiree'对这些植物激素对生长,蛋白质含量和抗氧化酶的改善潜力。的0 A 4×3因子组合,40,60,或80 mM的氯化钠与0,7或14μMIAA,或0,14或21μMGA 3将其加入Murashige和Skoog(MS)基础培养基中,然后接种结节外植体或愈伤组织培养物。记录以下数据:根和芽的数量和长度,节和叶的数量,植物的新鲜重量,愈伤组织培养物的新鲜重量的增加或减少,总可溶性蛋白以及超氧化物歧化酶(SOD)和过氧化物酶(POD)活性的数据。 30天 与对照相比,遭受NaCl胁迫的愈伤组织培养物和结外植体的生长均显着降低。IAA和GA 3均成功缓解了盐胁迫对研究的所有生长参数的有害影响。盐胁迫导致蛋白质含量降低,当培养基中也含有植物激素时,蛋白质含量会增加。IAA或GA均可增加SOD和POD的活性3在NaCl胁迫下。因此,IAA和GA 3的外源施用不仅在体外马铃薯生长方面发挥了积极作用,而且显着影响了测试的生化参数。

更新日期:2020-01-13
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