A novel procedure was developed for the determination of DCPTA residues in mung bean using dispersive solid-phase extraction (DSPE) couple with ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS). Sample was extracted with a HAc-acetonitrile solution (1:99, V/V) and primarily purified by DSPE with C₁₈, PSA, and GCB as absorbent. In order to detect DCPTA residues from mung bean, retention time, quantitative ions, and qualitative ions were used for screening and confirmation. The effect DSPE conditions of the type and dosage of adsorbents and extraction condition were researched, and then the suitable method was founded. Under the optimized conditions, the detection limit (S/N = 3) and quantitative limit (S/N = 10) of DCPTA were 0.11 μg kg⁻¹ and 0.37 μg kg⁻¹, respectively. The average recoveries ranged from 93.8 to 96.8% at spiking levels of 1.0, 5.0, and 10.0 μg kg⁻¹, with a relative standard deviation (RSD) lower than 6.2% (N = 6). This method is simple, accurate, and sensitive, and is suitable for the rapid screening and confirmatory analysis of plant growth regulator residues in coarse grains.

Graphical abstract

建立了一种使用分散固相萃取（DSPE）结合超高效液相色谱-串联质谱（UHPLC-MS / MS）测定绿豆中DCPTA残留的新方法。样品用HAc-乙腈溶液（1：99，V / V）萃取，并通过DSPE以C ₁₈，PSA和GCB作为吸收剂进行初步纯化。为了检测绿豆中的DCPTA残留，使用保留时间，定量离子和定性离子进行筛选和确认。研究了DSPE条件对吸附剂类型，用量和萃取条件的影响，建立了合适的方法。在最佳条件下，DCPTA的检出限（S / N = 3）和定量限（S / N = 10）为0.11μgkg ^-1和分别为0.37μgkg ^-1。在1.0、5.0和10.0μgkg ^-1的加标水平下，平均回收率介于93.8至96.8％之间，相对标准偏差（RSD）低于6.2％（N = 6）。该方法简单，准确，灵敏，适用于粗粮中植物生长调节剂残留的快速筛选和确证分析。

图形概要